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Cosmids allow you to insert DNA sequences (genes) into the genome of a bacteria, much like a plasmid. Cosmids in recombinant DNA technology, however, utilize phage (bacterial viruses) to enable researchers to insert DNA sequences of genes with up to 44,000 base pairs, while normal plasmids are only able to efficiently carry 10,000 base pairs. This allows researchers to insert more complex genes into bacteria as well as increases the efficiency with which researchers can make genomic libraries of more complex organisms.
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What is the difference between cosmid and plasmid?
A cosmid is a type of hybrid vector that combines features of a plasmid and a bacteriophage. It can carry larger DNA fragments (up to 45kb) compared to plasmids (up to 10kb). Cosmids have a plasmid backbone for replication in bacteria and a phage attachment site for packaging DNA into phage particles for transfer into host cells.
When did DNA Plant Technology end?
DNA Plant Technology ended in 2002.
What is the role of Cloning Host in recombinant DNA technology?
The Cloning Host is a cell that carries a recombinant DNA molecule and replicates it to produce multiple copies. It plays a crucial role in amplifying the desired DNA fragment before it can be studied or used for further experiments. E. coli is a common host organism used in recombinant DNA technology due to its fast growth rate and well-characterized genetics.
Explain the role of klenow fragment in recombinant DNA technology?
The Klenow fragment, derived from the DNA polymerase I enzyme, is used in recombinant DNA technology to fill in the single-stranded DNA gaps left in a vector after annealing with a DNA insert. It possesses 5' to 3' polymerase activity and 3' to 5' exonuclease activity, allowing it to extend the DNA strands in a template-directed manner. This helps to create recombinant DNA molecules with high efficiency.
The role of restriction enzymes in DNA technology is to?
Produce a cut (usually staggered) at a specific recognition sequences on DNA.
Circular DNA molecule that is not part of the chromosome in bacterial cells?
The circular DNA molecule that is not part of the bacterial chromosome is called a plasmid. Plasmids are small, extrachromosomal pieces of DNA that can replicate independently of the bacterial chromosome. They often carry genes that provide bacteria with additional functions, such as antibiotic resistance.
What role does E. coli play in recombinant DNA Technology?
It helps to create human insulin.
What is the difference between cosmid and plasmid?
A cosmid is a type of hybrid vector that combines features of a plasmid and a bacteriophage. It can carry larger DNA fragments (up to 45kb) compared to plasmids (up to 10kb). Cosmids have a plasmid backbone for replication in bacteria and a phage attachment site for packaging DNA into phage particles for transfer into host cells.
What is difference between recombinant DNA technology and Polymerase chain reaction?
r DNA technology is technology of creating new combination of DNA. While pcr is one of techniques used in r DNA technology for amplification of perticuler DNA fragment
When did DNA Plant Technology end?
DNA Plant Technology ended in 2002.
What is the role of Cloning Host in recombinant DNA technology?
The Cloning Host is a cell that carries a recombinant DNA molecule and replicates it to produce multiple copies. It plays a crucial role in amplifying the desired DNA fragment before it can be studied or used for further experiments. E. coli is a common host organism used in recombinant DNA technology due to its fast growth rate and well-characterized genetics.
Explain the role of klenow fragment in recombinant DNA technology?
The Klenow fragment, derived from the DNA polymerase I enzyme, is used in recombinant DNA technology to fill in the single-stranded DNA gaps left in a vector after annealing with a DNA insert. It possesses 5' to 3' polymerase activity and 3' to 5' exonuclease activity, allowing it to extend the DNA strands in a template-directed manner. This helps to create recombinant DNA molecules with high efficiency.
The role of restriction enzymes in DNA technology is to?
Produce a cut (usually staggered) at a specific recognition sequences on DNA.
Which best explains why DNA technology is important for disease prevention?
DNA technology allows for the identification of genetic predispositions to diseases, enabling personalized medicine and targeted interventions for at-risk individuals. It also facilitates the development of precise diagnostic tests for early detection of diseases and monitoring treatment responses. Furthermore, DNA technology plays a crucial role in developing vaccines and therapeutics by understanding the genetic basis of diseases.
Importance of limitation enzyme action in the technology of DNA recombinant?
Limitation enzymes are crucial in DNA recombinant technology because they can precisely cut DNA at specific sequences, allowing for the removal or insertion of genes. This enables the creation of recombinant DNA molecules with desired genetic information. Limitation enzymes play a key role in gene cloning, gene editing, and other biotechnological applications.
How does DNA technology transfer bacteria genes from cell to cell?
DNA technology will transfer bacteria genes from cell to cell.
What career combines DNA technology and medicine?
Pharmaceuticals combine DNA technology and medicine.
