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What is virtual cDNA library?
I imagine its just an online cDNA library. A cDNA library is of course a collection of cDNA copy sequences. cDNA is where you have mRNA and you use reverse transcriptase to turn a strand of RNA into a DNA equivalent, then use RNAase H to degrade the remaining RNA strand and then use DNA polymerase to create a complete double stranded DNA sequence that is the equivalent of the mRNA. This way you can get the gene without the introns that normal DNA would have.
What is a collection of cells containing DNA fragments produced by restriction enzymes and incorporated into plasmids called?
A DNA LibraryA collection of cells containing DNA fragments produced by restriction enzymes and incorporated into plasmids is called a DNA library. RNA can manufacture DNA via the action of reverse transcriptase.
What is the importance of DNA transcrip?
DNA transcrip transcribes the DNA so that RNA may use it and replicate it.
A collection of host cells that house different cloned fragments of DNA?
A bacterial artificial chromosome (BAC) library is a collection of host cells, typically E. coli, that each contain a clone of a different fragment of DNA. These host cells are used for the storage and propagation of these cloned DNA fragments for various genetic and molecular biology studies.
A set of bacterial clones collectively have fragments representing all of the DNA from another organism in them is called a?
genomic library, a set clones that collectively contain all of the DNA in an organis's genome
What is virtual cDNA library?
I imagine its just an online cDNA library. A cDNA library is of course a collection of cDNA copy sequences. cDNA is where you have mRNA and you use reverse transcriptase to turn a strand of RNA into a DNA equivalent, then use RNAase H to degrade the remaining RNA strand and then use DNA polymerase to create a complete double stranded DNA sequence that is the equivalent of the mRNA. This way you can get the gene without the introns that normal DNA would have.
What is Rapid Library used for?
The GS FLX Titanium Rapid Library Preparation Kit is used for processing a DNA sample into a library that contains single-stranded fragments of DNA for amplification using emPCR kits.
How is cell's DNA like the books in the library?
Cell's are constructed and branched into smaller categories like a library
What is a collection of cells containing DNA fragments produced by restriction enzymes and incorporated into plasmids called?
A DNA LibraryA collection of cells containing DNA fragments produced by restriction enzymes and incorporated into plasmids is called a DNA library. RNA can manufacture DNA via the action of reverse transcriptase.
The DNA fragments making up a genomic library are generally contained where?
The DNA fragments making up a genomic library are generally contained within bacterial or viral vectors, such as plasmids or bacteriophages. These vectors are used to transform host cells, where the DNA fragments can be replicated and stored as part of the library.
Difference between genomic library and cdna library?
A cDNA (complementary DNA) library is a DNA library that has been created from mRNAs that are present in the cell. Since a cDNA is created from mRNA transcripts, that means that in Eukaryotic organisms there will be no introns or transcriptional factors present in the cDNA library, only exons. Only protein coding regions will be present in a cDNA library. This also means that a cDNA library is often times tissue specific. Since the expression of mRNAs will be different in different tissues of the organism it will appear different then a genomic library. Often times to offset this problem a cDNA library will be composed of different tissues (brain, liver, heart) to encompass a greater variety of the proteins that are expressed. A genomic library will contain all the exons, introns, and transcriptional factors that are not found in the cDNA library. **2/24/2011** cDNA library does contain exons, which is the protein coding regions.
What is the complete collection of cloned DNA fragments from an organism?
A genomic DNA library is the complete collection of cloned DNA fragments from an organism, representing its entire genome. These libraries are used for various molecular biology applications, such as gene cloning, sequencing, and functional analysis.
What organelle is similar to the public library?
The nucleus is similar to a library as it contains sorted information (DNA) accessible to the cell.
What is a cDNA library used for?
A cDNA library is used for complementary DNA. These DNA are collected as host cells, which can be found in the nucleus. Currently, cDNA libraries are lacking in the enhancer, intron, and several other categories.
How is a genomic library produced?
A genomic library is produced by isolating DNA from an organism, fragmenting it into smaller pieces, and inserting these fragments into a vector (such as a plasmid or a phage). The vector is then introduced into a host organism, such as bacteria, which will replicate the DNA fragments along with their own DNA, creating a library of the organism's entire genome.
How do scientist locate a specific gene in a genomic library?
Scientists use a nucleic acid probe where scientists treat the DNA being searched with chemicals or heat to seperate the two DNA strands, then the nucleic acid probe is mixed with the single strands and the probe tags the direct DNA portion.
Do scientists use real DNA?
Yes, they do use real DNA.
