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A cDNA (complementary DNA) library is a DNA library that has been created from mRNAs that are present in the cell. Since a cDNA is created from mRNA transcripts, that means that in Eukaryotic organisms there will be no introns or transcriptional factors present in the cDNA library, only exons. Only protein coding regions will be present in a cDNA library. This also means that a cDNA library is often times tissue specific. Since the expression of mRNAs will be different in different tissues of the organism it will appear different then a genomic library. Often times to offset this problem a cDNA library will be composed of different tissues (brain, liver, heart) to encompass a greater variety of the proteins that are expressed. A genomic library will contain all the exons, introns, and transcriptional factors that are not found in the cDNA library.
**2/24/2011**
cDNA library does contain exons, which is the protein coding regions.
Wiki User
∙ 13y ago
Wiki User
∙ 11y agoOne of the most primary difference is in the structure of DNA, Genomic DNA is in linear from and Plasmids are in Circular form, then Secondly the Genomic DNA belongs to Eukaryote and Plasmids are Bacterial or prokaryotic DNA, which having no specific role in the inheritance of Bacteria.
Wiki User
∙ 15y agoThere is no difference. They refer to the same thing
Wiki User
∙ 11y agoGenomic library contains the total DNA of a particular organism, where in cDNA library contains only coding sequences of expressed proteins.
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Why you chose mRNA rather than genomic DNA for making a DNA library?
Complementary DNA (cDNA) is a doublestranded DNA version of RNA . Messenger RNA is a more useful predictor of a polypeptide sequence than DNA, because the introns have been spliced out. Scientists use cDNA rather than mRNA itself because RNAs are less stable than DNA.
CDNA is copied from?
mRNA
A strand of mRNA has the bases guanine-adenine-uracil Which amino acid corresponds to these bases?
Asp
What is the use of DNA library?
cDNA is the short form complementary DNA. cDNA libraries are a combination of cloned cDNA fragments. cDNA libraries are used to express eukaryotic genes in prokaryotes.
Why you chose mRNA rather than genomic DNA for making a DNA library?
Complementary DNA (cDNA) is a doublestranded DNA version of RNA . Messenger RNA is a more useful predictor of a polypeptide sequence than DNA, because the introns have been spliced out. Scientists use cDNA rather than mRNA itself because RNAs are less stable than DNA.
What is the advantage of using cDNA library in molecular genetics?
The main advantage of cDNA library is that it contains only the coding region of a genome.
Does a cDNA library have only exons?
A cDNA library consists only of genes that are expressed, hence they do contain only exons. They contain no introns.
What is a cDNA library used for?
A cDNA library is used for complementary DNA. These DNA are collected as host cells, which can be found in the nucleus. Currently, cDNA libraries are lacking in the enhancer, intron, and several other categories.
What is virtual cDNA library?
I imagine its just an online cDNA library. A cDNA library is of course a collection of cDNA copy sequences. cDNA is where you have mRNA and you use reverse transcriptase to turn a strand of RNA into a DNA equivalent, then use RNAase H to degrade the remaining RNA strand and then use DNA polymerase to create a complete double stranded DNA sequence that is the equivalent of the mRNA. This way you can get the gene without the introns that normal DNA would have.
What is a genomic library?
Specific human genes stored in virus, bacteria and yeast hosts no, the genes are randomly inserted into vectors. a cDNA library houses tissue-specific sequences derived from an mRNA transcript so that it contains only genes that code for protein.
What is genomic chips?
Genome chips are miniaturized plates containing hundreds of microscopic wells on their surface. These wells contain DNA probes. DNA probes are basically stretches of cDNA from a particular genome. When genomic DNA isolated from an organism is allowed to interact with the cDNA probes, come probes bind to the genomic DNA while others do not (depending on complementarity. A laser light is used to read each well and look at what sequences are bound. This information is valuable to scientists who can determine changes in gene expression based on the information obtained from a gene chip
What are the basic steps during obtaining a target gene from the cDNA library?
The advantage of cDNA library is that it contains only the coding region of a genome. To prepare a cDNA library, the first step is to isolate the total mRNA from the cell type of interest.Then the enzyme reverse transcriptase is used to synthesize a DNA strand complementary to each mRNA molecule. After the single-stranded DNA molecules are converted into double-stranded DNA molecules by DNA polymerase, they are inserted into vectors and cloned.
What is the full form of cdna?
CDNA = Complimentary Deoxyribose Nucleic Acid
What is a DNA tiling array?
A DNA tiling array uses overlapping DNA fragments. Hundreds of thousands of genomic fragments can be can be spotted onto a glass slide. In most tiling arrays, RNA samples are still hybridized to the slide just like a cDNA array.
What is difference between Bac arrays and DNA arrays?
BAC (Bacterial Artificial Chromosome) arrays are a type of DNA arrays. BAC arrays are usually used for a technique called array CGH (Comparative Genomic Hybridisation) which is used to identify gross deletions or amplifications in DNA (which for example is common in cancer). DNA arrays include BAC arrays but also oligo, cDNA, and promoter arrays. Oligo and cDNA arrays are typically used for gene expression analysis (looking to see how heavily expressed each gene is). Oligo arrays can also be used for SNP (single nucleotide polymorphism) analysis. Promoter arrays are used to identify transcription factor binding sites.
