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A cDNA (complementary DNA) library is a DNA library that has been created from mRNAs that are present in the cell. Since a cDNA is created from mRNA transcripts, that means that in Eukaryotic organisms there will be no introns or transcriptional factors present in the cDNA library, only exons. Only protein coding regions will be present in a cDNA library. This also means that a cDNA library is often times tissue specific. Since the expression of mRNAs will be different in different tissues of the organism it will appear different then a genomic library. Often times to offset this problem a cDNA library will be composed of different tissues (brain, liver, heart) to encompass a greater variety of the proteins that are expressed. A genomic library will contain all the exons, introns, and transcriptional factors that are not found in the cDNA library.
**2/24/2011**
cDNA library does contain exons, which is the protein coding regions.
What else can I help you with?
Why you chose mRNA rather than genomic DNA for making a DNA library?
Complementary DNA (cDNA) is a doublestranded DNA version of RNA . Messenger RNA is a more useful predictor of a polypeptide sequence than DNA, because the introns have been spliced out. Scientists use cDNA rather than mRNA itself because RNAs are less stable than DNA.
CDNA is copied from?
mRNA
A strand of mRNA has the bases guanine-adenine-uracil Which amino acid corresponds to these bases?
Asp
Does a cDNA library have only exons?
Yes, a cDNA library contains only exons because it is generated from mRNA, which has had introns removed through the process of splicing. cDNA represents the expressed regions of the genome and does not contain non-coding introns found in genomic DNA.
Does cDNA have a promoter region?
Yes, cDNA does not have a promoter region because it is synthesized from mRNA and lacks the regulatory elements found in genomic DNA.
Can clones isolated from cDNA libraries contain promoter sequences?
No, clones isolated from cDNA libraries do not contain promoter sequences because the cDNA synthesis process does not retain regulatory elements such as promoters. cDNA is made from mature mRNA and lacks the non-coding regions found in genomic DNA, including promoters. Therefore, clones isolated from cDNA libraries do not include promoter sequences.
Why you chose mRNA rather than genomic DNA for making a DNA library?
Complementary DNA (cDNA) is a doublestranded DNA version of RNA . Messenger RNA is a more useful predictor of a polypeptide sequence than DNA, because the introns have been spliced out. Scientists use cDNA rather than mRNA itself because RNAs are less stable than DNA.
What is the advantage of using cDNA library in molecular genetics?
The main advantage of cDNA library is that it contains only the coding region of a genome.
What is a cDNA library used for?
A cDNA library is used for complementary DNA. These DNA are collected as host cells, which can be found in the nucleus. Currently, cDNA libraries are lacking in the enhancer, intron, and several other categories.
What is the use of DNA library?
A DNA library is a collection of DNA fragments that represents the entire genome of an organism. It is used in genetic research to identify specific genes, study gene expression patterns, and clone genes of interest. DNA libraries are also used in techniques such as genome sequencing and gene mapping.
What is a genomic library?
Specific human genes stored in virus, bacteria and yeast hosts no, the genes are randomly inserted into vectors. a cDNA library houses tissue-specific sequences derived from an mRNA transcript so that it contains only genes that code for protein.
What are the differences between CDs and CDNA?
CDs, or certificates of deposit, are financial products offered by banks that pay a fixed interest rate for a specific term. CDNA, on the other hand, stands for cDNA, which is a type of DNA that is synthesized from a messenger RNA template. The main difference is that CDs are a financial investment, while cDNA is a type of genetic material used in molecular biology research.
What is virtual cDNA library?
I imagine its just an online cDNA library. A cDNA library is of course a collection of cDNA copy sequences. cDNA is where you have mRNA and you use reverse transcriptase to turn a strand of RNA into a DNA equivalent, then use RNAase H to degrade the remaining RNA strand and then use DNA polymerase to create a complete double stranded DNA sequence that is the equivalent of the mRNA. This way you can get the gene without the introns that normal DNA would have.
The function of cdna?
hi In vitro we must converted the RNA to cDNA to diagnosis viral RNA in PCR. In vivo RNa viral infected the cell RNA converted to cDNA IN SIDE THE CELL BY REVERSE TRANSCRIPTASE therfore cDNA insertion in the DNA of cell infected thank you hi In vitro we must converted the RNA to cDNA to diagnosis viral RNA in PCR. In vivo RNa viral infected the cell RNA converted to cDNA IN SIDE THE CELL BY REVERSE TRANSCRIPTASE therfore cDNA insertion in the DNA of cell infected thank you
What is genomic chips?
Genome chips are miniaturized plates containing hundreds of microscopic wells on their surface. These wells contain DNA probes. DNA probes are basically stretches of cDNA from a particular genome. When genomic DNA isolated from an organism is allowed to interact with the cDNA probes, come probes bind to the genomic DNA while others do not (depending on complementarity. A laser light is used to read each well and look at what sequences are bound. This information is valuable to scientists who can determine changes in gene expression based on the information obtained from a gene chip
