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hi
In vitro we must converted the RNA to cDNA to diagnosis viral RNA in PCR.
In vivo RNa viral infected the cell RNA converted to cDNA IN SIDE THE CELL BY REVERSE TRANSCRIPTASE therfore cDNA insertion in the DNA of cell infected
thank you
hi
In vitro we must converted the RNA to cDNA to diagnosis viral RNA in PCR.
In vivo RNa viral infected the cell RNA converted to cDNA IN SIDE THE CELL BY REVERSE TRANSCRIPTASE therfore cDNA insertion in the DNA of cell infected
thank you
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∙ 12y ago
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Does a cDNA library have only exons?
A cDNA library consists only of genes that are expressed, hence they do contain only exons. They contain no introns.
A scientist wants to be able to artificially produce a protein that is produced in the livers of frogs. The scientist has located all of the mRNA produced by the liver and produced the cDNA. What shou?
Mix the cDNA with the liver DNA
What does c in CDna stand for?
There is no letter C in DNA or RNA .
How is a human gene recombined into a bacterial plasmid?
One of the most common ways these days is from cDNA. RNA is extracted from human cells, purified, and then treated with an enzyme called reverse transcriptase which is able to make DNA from RNA templates (this DNA made from RNA is called cDNA). The advantage of using cDNA is that in the genome human genes are typically distributed across multiple exons spread over tens or even hundreds of thousands of basepairs of DNA. Such a massive segment of DNA is extremely hard to manipulate and far too large to insert into a plasmid. However, in cDNA, all the introns have been spliced out (because cDNA is made from mature mRNA). To isolate a particular gene from cDNA, PCR is often used to selectively amplify one gene's cDNA using specific primers. To insert the amplified cDNA into a plasmid, the traditional approach was to use restriction enzymes - enzymes that cut precise DNA sequences. The great thing about many restriction enzymes is that they cut DNA but leave behind "sticky ends". Thus if you cut both your cDNA and a plasmid with a particular restriction enzyme, the resulting sticky ends will allow the human cDNA to be taken up by the plasmid (the sticky ends will mesh). The sticky ends will have to be sealed by an enzyme called DNA ligase. However, there are other ways these days - often involving recombination to insert the PCR product directly into a plasmid without resorting to restriction enzymes and ligations.
What should scientist do to find gene that produces protein after he located all the mrna produced by liver and cdna if he wants to artifically produce protein that is produced in liver of frogs?
The scientist should mix the cDNA with the liver DNA.
What is the full form of cdna?
CDNA = Complimentary Deoxyribose Nucleic Acid
CDNA is copied from?
mRNA
What is a cDNA library used for?
A cDNA library is used for complementary DNA. These DNA are collected as host cells, which can be found in the nucleus. Currently, cDNA libraries are lacking in the enhancer, intron, and several other categories.
What is the advantage of using cDNA library in molecular genetics?
The main advantage of cDNA library is that it contains only the coding region of a genome.
What is the use of DNA library?
cDNA is the short form complementary DNA. cDNA libraries are a combination of cloned cDNA fragments. cDNA libraries are used to express eukaryotic genes in prokaryotes.
What is virtual cDNA library?
I imagine its just an online cDNA library. A cDNA library is of course a collection of cDNA copy sequences. cDNA is where you have mRNA and you use reverse transcriptase to turn a strand of RNA into a DNA equivalent, then use RNAase H to degrade the remaining RNA strand and then use DNA polymerase to create a complete double stranded DNA sequence that is the equivalent of the mRNA. This way you can get the gene without the introns that normal DNA would have.
Does a cDNA library have only exons?
A cDNA library consists only of genes that are expressed, hence they do contain only exons. They contain no introns.
What is the purpose of the cDNA synthesis?
The purpose of cDNA synthesis is to synthesize a copy of DNA from mRNA. This means that it is involved in the duplication of DNA that occurs when a cell divides. As a result, without cDNA synthesis, life would not exist as cells would not be able to divide.
Which processes are involved in making cDNA?
translation
What is the meaning of CDNA microarray?
A cDNA microarray is a hybrid of a DNA microarray, which is a collection of a number of minute DNA dots. These are mostly used in the field of genetic testing.
What is extracted from cells for DNA microarray technology?
mRNA is extracted from cells for DNA microarray. the mRNA is then converted in the lab to cDNA this cDNA is allowed to interact with the probes on the microarray chip
How is cdNA created?
cDNA or complimentary DNA is created by a catalyzed reaction from DNA polymerase and reverse transcriptase which results in the use of mRNA or messenger RNA as template for DNA synthesis.
