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In vitro we must converted the RNA to cDNA to diagnosis viral RNA in PCR.

In vivo RNa viral infected the cell RNA converted to cDNA IN SIDE THE CELL BY REVERSE TRANSCRIPTASE therfore cDNA insertion in the DNA of cell infected

thank you

hi

In vitro we must converted the RNA to cDNA to diagnosis viral RNA in PCR.

In vivo RNa viral infected the cell RNA converted to cDNA IN SIDE THE CELL BY REVERSE TRANSCRIPTASE therfore cDNA insertion in the DNA of cell infected

thank you

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13y ago

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Related Questions

Is cDNA complementary to mRNA?

Yes, cDNA is complementary to mRNA.


What is the full form of cdna?

CDNA = Complimentary Deoxyribose Nucleic Acid


How can I create cDNA in the laboratory?

To create cDNA in the laboratory, you can follow these steps: Extract RNA from the cells or tissue of interest. Use reverse transcriptase enzyme to convert RNA into cDNA. Purify and amplify the cDNA using PCR (polymerase chain reaction). Verify the cDNA sequence through sequencing techniques.


CDNA is copied from?

mRNA


What is a cDNA library used for?

A cDNA library is used for complementary DNA. These DNA are collected as host cells, which can be found in the nucleus. Currently, cDNA libraries are lacking in the enhancer, intron, and several other categories.


What is the advantage of using cDNA library in molecular genetics?

The main advantage of cDNA library is that it contains only the coding region of a genome.


Does cDNA have a promoter region?

Yes, cDNA does not have a promoter region because it is synthesized from mRNA and lacks the regulatory elements found in genomic DNA.


What is the purpose of the cDNA synthesis?

The purpose of cDNA synthesis is to synthesize a copy of DNA from mRNA. This means that it is involved in the duplication of DNA that occurs when a cell divides. As a result, without cDNA synthesis, life would not exist as cells would not be able to divide.


Which processes are involved in making cDNA?

translation


What statement best compares the base sequence of an mRNA molecule with that of the cDNA made from the mRNA?

The base sequence of cDNA is complementary to the mRNA molecule from which it is synthesized. This means that the cDNA will have the same sequence as the mRNA, except that thymine in DNA is replaced with uracil in RNA.


Can clones isolated from cDNA libraries contain promoter sequences?

No, clones isolated from cDNA libraries do not contain promoter sequences because the cDNA synthesis process does not retain regulatory elements such as promoters. cDNA is made from mature mRNA and lacks the non-coding regions found in genomic DNA, including promoters. Therefore, clones isolated from cDNA libraries do not include promoter sequences.


Does a cDNA library have only exons?

Yes, a cDNA library contains only exons because it is generated from mRNA, which has had introns removed through the process of splicing. cDNA represents the expressed regions of the genome and does not contain non-coding introns found in genomic DNA.