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What statement best compares the base sequence of an mRNA molecule with that of the cDNA made from the mRNA?
The base sequence of cDNA is complementary to the mRNA molecule from which it is synthesized. This means that the cDNA will have the same sequence as the mRNA, except that thymine in DNA is replaced with uracil in RNA.
What does c in CDna stand for?
The "c" in cDNA stands for complementary. cDNA is synthesized from mRNA using reverse transcriptase, resulting in a complementary DNA strand that lacks introns and represents the protein-coding regions of a gene.
What is formed when reverse transcriptase is used on a strand of mRNA?
A strand of DNA
In order for a bacterium to produce a eukaryotic protein what must first be isolated from the cell prior to cloning?
The cDNA (complementary DNA) encoding the eukaryotic protein must first be isolated from the cell prior to cloning. This involves reverse transcription of the messenger RNA (mRNA) and subsequent amplification to obtain the gene of interest for cloning into a bacterial expression vector.
Does cDNA have a promoter region?
Yes, cDNA does not have a promoter region because it is synthesized from mRNA and lacks the regulatory elements found in genomic DNA.
What statement best compares the base sequence of an mRNA molecule with that of the cDNA made from the mRNA?
The base sequence of cDNA is complementary to the mRNA molecule from which it is synthesized. This means that the cDNA will have the same sequence as the mRNA, except that thymine in DNA is replaced with uracil in RNA.
What does c in CDna stand for?
The "c" in cDNA stands for complementary. cDNA is synthesized from mRNA using reverse transcriptase, resulting in a complementary DNA strand that lacks introns and represents the protein-coding regions of a gene.
In genetics what does complementary DNA mean?
Complementary DNA (cDNA) is DNA that has been copied from an mRNA through a reverse transcriptase enzyme. cDNA contains a copy of the original DNA sequence that made the mRNA - but without the introns (as these are cut out to create mRNA).
Why doesnt cdna have introns?
cDNA, or complementary DNA, is synthesized from mature mRNA through the process of reverse transcription. During this process, introns are removed from the pre-mRNA through splicing, resulting in a continuous coding sequence. Since cDNA is derived from this spliced mRNA, it contains only exons, which are the coding regions necessary for protein synthesis, and thus lacks introns.
How is cdNA created?
cDNA (complementary DNA) is created by using an enzyme called reverse transcriptase to convert messenger RNA (mRNA) into a complementary DNA strand. This process, known as reverse transcription, results in a single-stranded cDNA molecule that can be further amplified and used for various molecular biology applications.
How did scientists convert information when using reverse transferiptase in insulin research?
Scientists used reverse transcriptase to convert messenger RNA (mRNA) into complementary DNA (cDNA) in insulin research. This cDNA copy of the mRNA can then be studied and amplified to better understand the genetic information that codes for insulin production. By converting the mRNA into cDNA, scientists are able to analyze and manipulate the genetic information more easily in their research.
CDNA is copied from?
mRNA
What is extracted from cells for DNA microarray technology?
mRNA is extracted from cells for DNA microarray. the mRNA is then converted in the lab to cDNA this cDNA is allowed to interact with the probes on the microarray chip
What is formed when reverse transcriptase is used on a strand of mRNA?
A strand of DNA
When is DNA produced from mRNA?
DNA is produced from mRNA during the process of reverse transcription, which occurs in retroviruses and certain cellular processes. In this process, an enzyme called reverse transcriptase synthesizes complementary DNA (cDNA) from an mRNA template. This mechanism is crucial for retroviral replication and is also utilized in molecular biology techniques, such as the creation of cDNA libraries.
What enzyme do you need to get a copy of DNA from mRNA?
The enzyme needed to make a DNA copy of RNA (including mRNA) is called reverse transcriptase. This enzyme is capable of synthesizing a complementary DNA strand from an RNA template, which is the first step in generating a cDNA (complementary DNA) library.
When is complementary DNA produced in the body?
Complementary DNA (cDNA) is produced in the body during the process of reverse transcription. This occurs primarily in retroviruses, where viral RNA is converted into cDNA by the enzyme reverse transcriptase. Additionally, cDNA can be synthesized in laboratory settings from mRNA for applications such as cloning, sequencing, and gene expression analysis. In the context of cellular processes, cDNA is not typically produced in normal cellular functions, as cells primarily use DNA for genetic information storage and RNA for protein synthesis.
