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mRNA
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Difference between genomic library and cdna library?
A cDNA (complementary DNA) library is a DNA library that has been created from mRNAs that are present in the cell. Since a cDNA is created from mRNA transcripts, that means that in Eukaryotic organisms there will be no introns or transcriptional factors present in the cDNA library, only exons. Only protein coding regions will be present in a cDNA library. This also means that a cDNA library is often times tissue specific. Since the expression of mRNAs will be different in different tissues of the organism it will appear different then a genomic library. Often times to offset this problem a cDNA library will be composed of different tissues (brain, liver, heart) to encompass a greater variety of the proteins that are expressed. A genomic library will contain all the exons, introns, and transcriptional factors that are not found in the cDNA library. **2/24/2011** cDNA library does contain exons, which is the protein coding regions.
Why you chose mRNA rather than genomic DNA for making a DNA library?
Complementary DNA (cDNA) is a doublestranded DNA version of RNA . Messenger RNA is a more useful predictor of a polypeptide sequence than DNA, because the introns have been spliced out. Scientists use cDNA rather than mRNA itself because RNAs are less stable than DNA.
What stage during the cell cycle are organelles and chromosomes copied?
i think its telophase
A strand of mRNA has the bases guanine-adenine-uracil Which amino acid corresponds to these bases?
Asp
What is the process of going from DNA to RNA called?
Transcription.During transcription the base sequence (genetic code) of part (a gene) of one strand of DNA is copied onto a strand of RNA as the RNA is synthesized.
What is the full form of cdna?
CDNA = Complimentary Deoxyribose Nucleic Acid
The function of cdna?
hi In vitro we must converted the RNA to cDNA to diagnosis viral RNA in PCR. In vivo RNa viral infected the cell RNA converted to cDNA IN SIDE THE CELL BY REVERSE TRANSCRIPTASE therfore cDNA insertion in the DNA of cell infected thank you hi In vitro we must converted the RNA to cDNA to diagnosis viral RNA in PCR. In vivo RNa viral infected the cell RNA converted to cDNA IN SIDE THE CELL BY REVERSE TRANSCRIPTASE therfore cDNA insertion in the DNA of cell infected thank you
What is a cDNA library used for?
A cDNA library is used for complementary DNA. These DNA are collected as host cells, which can be found in the nucleus. Currently, cDNA libraries are lacking in the enhancer, intron, and several other categories.
What is the advantage of using cDNA library in molecular genetics?
The main advantage of cDNA library is that it contains only the coding region of a genome.
In genetics what does complementary DNA mean?
Complementary DNA (cDNA) is DNA that has been copied from an mRNA through a reverse transcriptase enzyme. cDNA contains a copy of the original DNA sequence that made the mRNA - but without the introns (as these are cut out to create mRNA).
What is the use of DNA library?
cDNA is the short form complementary DNA. cDNA libraries are a combination of cloned cDNA fragments. cDNA libraries are used to express eukaryotic genes in prokaryotes.
What is virtual cDNA library?
I imagine its just an online cDNA library. A cDNA library is of course a collection of cDNA copy sequences. cDNA is where you have mRNA and you use reverse transcriptase to turn a strand of RNA into a DNA equivalent, then use RNAase H to degrade the remaining RNA strand and then use DNA polymerase to create a complete double stranded DNA sequence that is the equivalent of the mRNA. This way you can get the gene without the introns that normal DNA would have.
Does a cDNA library have only exons?
A cDNA library consists only of genes that are expressed, hence they do contain only exons. They contain no introns.
What is the purpose of the cDNA synthesis?
The purpose of cDNA synthesis is to synthesize a copy of DNA from mRNA. This means that it is involved in the duplication of DNA that occurs when a cell divides. As a result, without cDNA synthesis, life would not exist as cells would not be able to divide.
Which processes are involved in making cDNA?
translation
What is the meaning of CDNA microarray?
A cDNA microarray is a hybrid of a DNA microarray, which is a collection of a number of minute DNA dots. These are mostly used in the field of genetic testing.
How is cdNA created?
cDNA or complimentary DNA is created by a catalyzed reaction from DNA polymerase and reverse transcriptase which results in the use of mRNA or messenger RNA as template for DNA synthesis.
