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cDNA or complimentary DNA is created by a catalyzed reaction from DNA polymerase and reverse transcriptase which results in the use of mRNA or messenger RNA as template for DNA synthesis.

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∙ 13y ago

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What is virtual cDNA library?

I imagine its just an online cDNA library. A cDNA library is of course a collection of cDNA copy sequences. cDNA is where you have mRNA and you use reverse transcriptase to turn a strand of RNA into a DNA equivalent, then use RNAase H to degrade the remaining RNA strand and then use DNA polymerase to create a complete double stranded DNA sequence that is the equivalent of the mRNA. This way you can get the gene without the introns that normal DNA would have.


CDNA is significant when?

cDNA is significant because it is a synthesized DNA copy of mRNA that lacks introns and contains only exons. This allows for the study of gene expression and regulation, as well as the production of recombinant proteins or gene therapy vectors. cDNA is particularly useful in understanding genetic diseases and developing new therapeutics.


What is extracted from cells for DNA microarray technology?

mRNA is extracted from cells for DNA microarray. the mRNA is then converted in the lab to cDNA this cDNA is allowed to interact with the probes on the microarray chip


What should the scientists do to find the gene that produces this protein?

Mix the cDNA with the liver DNA.


What is c-DNA library and How it is constructed from a RNA?

c-DNA library is a combination of cloned c-DNA(complementary DNA)fragments inserted into a collection of host cells which together constitute some portion of transcriptome(it is a set of all RNA molecules including m-RNA,r-RNA,t-RNA and other non-coding RNA produced in one or a population of cells) of an organism.c-DNA is produced from fully transcribed m-RNA found in the nucleus and therefore contains only the expressed genes of an organism.

Related Questions

What is the full form of cdna?

CDNA = Complimentary Deoxyribose Nucleic Acid


Difference between genomic library and cdna library?

A cDNA (complementary DNA) library is a DNA library that has been created from mRNAs that are present in the cell. Since a cDNA is created from mRNA transcripts, that means that in Eukaryotic organisms there will be no introns or transcriptional factors present in the cDNA library, only exons. Only protein coding regions will be present in a cDNA library. This also means that a cDNA library is often times tissue specific. Since the expression of mRNAs will be different in different tissues of the organism it will appear different then a genomic library. Often times to offset this problem a cDNA library will be composed of different tissues (brain, liver, heart) to encompass a greater variety of the proteins that are expressed. A genomic library will contain all the exons, introns, and transcriptional factors that are not found in the cDNA library. **2/24/2011** cDNA library does contain exons, which is the protein coding regions.


CDNA is copied from?

mRNA


The function of cdna?

hi In vitro we must converted the RNA to cDNA to diagnosis viral RNA in PCR. In vivo RNa viral infected the cell RNA converted to cDNA IN SIDE THE CELL BY REVERSE TRANSCRIPTASE therfore cDNA insertion in the DNA of cell infected thank you hi In vitro we must converted the RNA to cDNA to diagnosis viral RNA in PCR. In vivo RNa viral infected the cell RNA converted to cDNA IN SIDE THE CELL BY REVERSE TRANSCRIPTASE therfore cDNA insertion in the DNA of cell infected thank you


Which processes are involved in making cDNA?

translation


Can clones isolated from cDNA libraries contain promoter sequences?

No, clones isolated from cDNA libraries do not contain promoter sequences because the cDNA synthesis process does not retain regulatory elements such as promoters. cDNA is made from mature mRNA and lacks the non-coding regions found in genomic DNA, including promoters. Therefore, clones isolated from cDNA libraries do not include promoter sequences.


Does a cDNA library have only exons?

Yes, a cDNA library contains only exons because it is generated from mRNA, which has had introns removed through the process of splicing. cDNA represents the expressed regions of the genome and does not contain non-coding introns found in genomic DNA.


What is virtual cDNA library?

I imagine its just an online cDNA library. A cDNA library is of course a collection of cDNA copy sequences. cDNA is where you have mRNA and you use reverse transcriptase to turn a strand of RNA into a DNA equivalent, then use RNAase H to degrade the remaining RNA strand and then use DNA polymerase to create a complete double stranded DNA sequence that is the equivalent of the mRNA. This way you can get the gene without the introns that normal DNA would have.


CDNA is significant when?

cDNA is significant because it is a synthesized DNA copy of mRNA that lacks introns and contains only exons. This allows for the study of gene expression and regulation, as well as the production of recombinant proteins or gene therapy vectors. cDNA is particularly useful in understanding genetic diseases and developing new therapeutics.


What is extracted from cells for DNA microarray technology?

mRNA is extracted from cells for DNA microarray. the mRNA is then converted in the lab to cDNA this cDNA is allowed to interact with the probes on the microarray chip


What is the use of DNA library?

A DNA library is a collection of DNA fragments that represents the entire genome of an organism. It is used in genetic research to identify specific genes, study gene expression patterns, and clone genes of interest. DNA libraries are also used in techniques such as genome sequencing and gene mapping.


What is formed when reverse transcriptase is used on a strand of mRNA?

A strand of DNA