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What else can I help you with?
In eukaryotes only exons are translated?
Correct. The mRNA transcibed from the DNA in the nucleus has both exons and introns; the introns are taken out and the exons are left in. The mended exons exit the nucleus and the introns stay in the nucleus. Only the exons are translated at the ribosomes. (In Eukaryotic cells only)
Can clones isolated from cDNA libraries contain promoter sequences?
No, clones isolated from cDNA libraries do not contain promoter sequences because the cDNA synthesis process does not retain regulatory elements such as promoters. cDNA is made from mature mRNA and lacks the non-coding regions found in genomic DNA, including promoters. Therefore, clones isolated from cDNA libraries do not include promoter sequences.
Is cDNA complementary to mRNA?
Yes, cDNA is complementary to mRNA.
How can I create cDNA in the laboratory?
To create cDNA in the laboratory, you can follow these steps: Extract RNA from the cells or tissue of interest. Use reverse transcriptase enzyme to convert RNA into cDNA. Purify and amplify the cDNA using PCR (polymerase chain reaction). Verify the cDNA sequence through sequencing techniques.
What happens to the introns and exons during transcription?
After transcription, the mRNA is processed by the spliceosome, which splices out the introns (because introns are not part of the coding sequences for protein), and "stitches" the exons together to form the final transcript that is sent to the ribosome for translation.
Difference between genomic library and cdna library?
A cDNA (complementary DNA) library is a DNA library that has been created from mRNAs that are present in the cell. Since a cDNA is created from mRNA transcripts, that means that in Eukaryotic organisms there will be no introns or transcriptional factors present in the cDNA library, only exons. Only protein coding regions will be present in a cDNA library. This also means that a cDNA library is often times tissue specific. Since the expression of mRNAs will be different in different tissues of the organism it will appear different then a genomic library. Often times to offset this problem a cDNA library will be composed of different tissues (brain, liver, heart) to encompass a greater variety of the proteins that are expressed. A genomic library will contain all the exons, introns, and transcriptional factors that are not found in the cDNA library. **2/24/2011** cDNA library does contain exons, which is the protein coding regions.
What is the advantage of using cDNA library in molecular genetics?
The main advantage of cDNA library is that it contains only the coding region of a genome.
CDNA is significant when?
cDNA is significant because it is a synthesized DNA copy of mRNA that lacks introns and contains only exons. This allows for the study of gene expression and regulation, as well as the production of recombinant proteins or gene therapy vectors. cDNA is particularly useful in understanding genetic diseases and developing new therapeutics.
What is a cDNA library used for?
A cDNA library is used for complementary DNA. These DNA are collected as host cells, which can be found in the nucleus. Currently, cDNA libraries are lacking in the enhancer, intron, and several other categories.
Why doesnt cdna have introns?
cDNA, or complementary DNA, is synthesized from mature mRNA through the process of reverse transcription. During this process, introns are removed from the pre-mRNA through splicing, resulting in a continuous coding sequence. Since cDNA is derived from this spliced mRNA, it contains only exons, which are the coding regions necessary for protein synthesis, and thus lacks introns.
What are the basic steps during obtaining a target gene from the cDNA library?
The advantage of cDNA library is that it contains only the coding region of a genome. To prepare a cDNA library, the first step is to isolate the total mRNA from the cell type of interest.Then the enzyme reverse transcriptase is used to synthesize a DNA strand complementary to each mRNA molecule. After the single-stranded DNA molecules are converted into double-stranded DNA molecules by DNA polymerase, they are inserted into vectors and cloned.
What genetic information does cDNA contain?
cDNA, or complementary DNA, contains the coding sequence of genes expressed in a specific cell type or tissue at a given time. It is synthesized from mRNA through the process of reverse transcription, meaning it reflects the transcriptome of the cell, including only the exons that are translated into proteins. cDNA does not contain introns or non-coding regions present in the genomic DNA. Therefore, it is useful for studying gene expression and for applications such as cloning and the production of recombinant proteins.
What is virtual cDNA library?
I imagine its just an online cDNA library. A cDNA library is of course a collection of cDNA copy sequences. cDNA is where you have mRNA and you use reverse transcriptase to turn a strand of RNA into a DNA equivalent, then use RNAase H to degrade the remaining RNA strand and then use DNA polymerase to create a complete double stranded DNA sequence that is the equivalent of the mRNA. This way you can get the gene without the introns that normal DNA would have.
What is c-DNA library and How it is constructed from a RNA?
c-DNA library is a combination of cloned c-DNA(complementary DNA)fragments inserted into a collection of host cells which together constitute some portion of transcriptome(it is a set of all RNA molecules including m-RNA,r-RNA,t-RNA and other non-coding RNA produced in one or a population of cells) of an organism.c-DNA is produced from fully transcribed m-RNA found in the nucleus and therefore contains only the expressed genes of an organism.
In eukaryotes only exons are translated?
Correct. The mRNA transcibed from the DNA in the nucleus has both exons and introns; the introns are taken out and the exons are left in. The mended exons exit the nucleus and the introns stay in the nucleus. Only the exons are translated at the ribosomes. (In Eukaryotic cells only)
What is the use of DNA library?
A DNA library is a collection of DNA fragments that represents the entire genome of an organism. It is used in genetic research to identify specific genes, study gene expression patterns, and clone genes of interest. DNA libraries are also used in techniques such as genome sequencing and gene mapping.
Can clones isolated from cDNA libraries contain promoter sequences?
No, clones isolated from cDNA libraries do not contain promoter sequences because the cDNA synthesis process does not retain regulatory elements such as promoters. cDNA is made from mature mRNA and lacks the non-coding regions found in genomic DNA, including promoters. Therefore, clones isolated from cDNA libraries do not include promoter sequences.
