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ELISA microplate readers operate by detecting and quantifying the optical signals generated during enzyme-linked immunosorbent assays (ELISAs). They measure the absorbance, fluorescence, or luminescence produced by a reaction between an enzyme-linked antibody and a substrate, which is proportional to the concentration of the target analyte. The reader uses light sources and detectors to measure the intensity of this signal, allowing for the determination of sample concentrations based on calibration curves. This process enables high-throughput analysis in a multi-well plate format.

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