DNA
Fred Griffith found that genetic information could be transferred between different strains of bacteria, specifically between the harmless R strain and the virulent S strain of bacteria. This led to the discovery of transformation, a process where genetic material is exchanged between bacteria.
Fred Griffith discovered that the harmless R strain of Streptococcus pneumoniae could be transformed into the deadly S strain when mixed with heat-killed S strain bacteria. This experiment provided evidence for the concept of bacterial transformation, where genetic material can be transferred between different strains of bacteria.
If you grow transformed bacteria containing a recombinant DNA plasmid without an antibiotic, you would likely observe that only a small proportion of the cells that successfully took up the plasmid will survive, while the majority of non-transformed cells will also grow. However, the transformed cells may not express the gene of interest or provide any selective advantage, resulting in no significant difference in growth compared to the non-transformed cells. Over time, the population would likely consist mostly of non-transformed cells, as they do not require any selective pressure to thrive.
The enzyme produced by cells transformed with plasmid lux that is not produced by cells transformed with pUC18 is luciferase. This enzyme is responsible for the bioluminescent properties of animals like fireflies and glowworms. Cells transformed with plasmid lux will emit light in the presence of the substrate luciferin, whereas cells transformed with pUC18 will not.
Smooth endoplasmic reticulum is a eukaryotic organelle found in eukaryotic cells, which includes all plant and animal cells. Prokaryotic cells, such as bacteria, lack membrane-bound organelles like the endoplasmic reticulum.
This experiment was conducted by Frederick Griffith in 1928. He observed that when he mixed a harmless strain of bacteria with a heat-killed virulent strain, the harmless bacteria were transformed into a virulent form. This led to the hypothesis that something in the heat-killed bacteria had transformed the live bacteria.
In Griffith's experiment, non-pathogenic bacteria were transformed into pathogenic bacteria by being exposed to heat-killed pathogenic bacteria, a process now known as transformation. This led to the discovery of genetic material transfer between bacteria.
No, bacteria do not have smooth endoplasmic reticulum (ER). Smooth ER is a cell organelle found in eukaryotic cells, not in prokaryotic cells like bacteria.
Griffith hypothesized that a chemical component from the virulent S cells had somehow transformed the R cells into the more virulent S form.
Fred Griffith found that genetic information could be transferred between different strains of bacteria, specifically between the harmless R strain and the virulent S strain of bacteria. This led to the discovery of transformation, a process where genetic material is exchanged between bacteria.
Fred Griffith discovered that the harmless R strain of Streptococcus pneumoniae could be transformed into the deadly S strain when mixed with heat-killed S strain bacteria. This experiment provided evidence for the concept of bacterial transformation, where genetic material can be transferred between different strains of bacteria.
Bacteria usually don't have all the organelles that plant and animal cells do, such as mitochondria, chloroplasts (plants), golgi apparatus, smooth and rough endoplasmic reticulums, etc. Bacteria cells are simple as compared to the plant and animal cells.
If you grow transformed bacteria containing a recombinant DNA plasmid without an antibiotic, you would likely observe that only a small proportion of the cells that successfully took up the plasmid will survive, while the majority of non-transformed cells will also grow. However, the transformed cells may not express the gene of interest or provide any selective advantage, resulting in no significant difference in growth compared to the non-transformed cells. Over time, the population would likely consist mostly of non-transformed cells, as they do not require any selective pressure to thrive.
An effector strain of bacteria is a strain that has the ability to produce and secrete specific proteins or molecules called effectors, which can manipulate host cells or immune responses to benefit the bacterium. These effectors can help the bacterium evade host defenses, establish infection, and promote survival within the host.
Bacteria usually don't have all the organelles that plant and animal cells do, such as mitochondria, chloroplasts (plants), golgi apparatus, smooth and rough endoplasmic reticulums, etc. Bacteria cells are simple as compared to the plant and animal cells.
The plasma cells develop from transformed b cells.
The enzyme produced by cells transformed with plasmid lux that is not produced by cells transformed with pUC18 is luciferase. This enzyme is responsible for the bioluminescent properties of animals like fireflies and glowworms. Cells transformed with plasmid lux will emit light in the presence of the substrate luciferin, whereas cells transformed with pUC18 will not.