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What are 2 important influences on enzymatic activity?
Heat and crupes
What shows enzymatic activity?
Enzymatic activity is demonstrated by the ability of enzymes to catalyze biochemical reactions. This can be observed by changes in substrate concentration, product formation, or by measuring activity using specific assays such as spectrophotometry or mass spectrometry. Additionally, enzyme activity can be modulated by factors such as pH, temperature, and the presence of cofactors or inhibitors.
What type of organic molecule may show enzymatic activity?
Enzymatic activity is primarily associated with proteins, which are organic molecules made up of amino acids. These proteins act as enzymes, catalyzing biochemical reactions by lowering activation energy. Some RNA molecules, known as ribozymes, can also exhibit enzymatic activity by catalyzing specific reactions, demonstrating that not only proteins but also certain nucleic acids can function as enzymes.
What are the substances needed for photosynthesis and what substances are produced?
CO2 and water is needed. Oxygen and glucose are produced
Macromolecules that can act as enzymes are?
Proteins are the primary macromolecules that can act as enzymes. They catalyze biochemical reactions by lowering the activation energy needed for the reaction to occur. Other macromolecules like RNA can also exhibit enzymatic activity, known as ribozymes.
What are 2 important influences on enzymatic activity?
Heat and crupes
What is autophosphorylation?
An autophosphorylation is the phosphorylation of a kinase protein catalyzed by its own enzymatic activity.
What shows enzymatic activity?
Enzymatic activity is demonstrated by the ability of enzymes to catalyze biochemical reactions. This can be observed by changes in substrate concentration, product formation, or by measuring activity using specific assays such as spectrophotometry or mass spectrometry. Additionally, enzyme activity can be modulated by factors such as pH, temperature, and the presence of cofactors or inhibitors.
When can refrigeration not applicable to preserve sample for enzymatic assay?
Refrigeration is not applicable to preserve sample for enzymatic assay because enzymes may lose their activity at extremely low temperatures as well. This may account for storing enzymes at 5° C or below without affecting the enzymatic activity permanently. (Anubhav, 2012)
What are the 2 conditions that destroy enzymatic activity by bond breakage?
1) Temperature 2)pH
Which substances are not needed for respiration?
Glucose and oxygen are needed for respiration. All other substances are not needed.
What type of organic molecule may show enzymatic activity?
Enzymatic activity is primarily associated with proteins, which are organic molecules made up of amino acids. These proteins act as enzymes, catalyzing biochemical reactions by lowering activation energy. Some RNA molecules, known as ribozymes, can also exhibit enzymatic activity by catalyzing specific reactions, demonstrating that not only proteins but also certain nucleic acids can function as enzymes.
What are some factors that may influence enzyme activity?
Factors affecting enzyme activity1: Enzyme concentrationIf the quantity of enzyme is doubled, the enzymatic activity will also be doubled because more enzymes are now available to work. After a certain level of enzyme concentration, there will be no more increase in the enzymatic activity because all the substrate molecules are combined with an enzyme and the rate of reaction will stabilize.2: Substrate concentrationBy increasing substrate concentration, enzymatic activity increases. Increasing the substrate further without increasing the enzyme concentration will not affect the enzymatic activity because all the enzymes are occupied by a substrate molecule.3: pH valueSome enzymes require acidic surroundings, most require a more neutral condition for their activity. Change in the pH can change the enzyme's structure and enzyme become useless.4: TemperatureAn increase in temperature of 10 degree celsius doubles the enzymatic activity. Each enzyme has its own optimum temperature at which its enzymatic activity is maximum. Very high temperatures break the bonds that maintain shape of enzyme. If the enzyme denatures, the substrate can not fit in to the active sites and enzyme become useless.
What is the recommended proteinase K buffer recipe for optimal enzymatic activity in a biological sample?
The recommended proteinase K buffer recipe for optimal enzymatic activity in a biological sample typically includes Tris-HCl, calcium chloride, and sodium chloride. This buffer helps maintain the stability and activity of proteinase K, an enzyme that breaks down proteins in the sample.
What are the substances needed for photosynthesis and what substances are produced?
CO2 and water is needed. Oxygen and glucose are produced
Explain the physiological significance of the differences in enzymatic activity under acidic pH?
The effects of low acidic pH in the differences of enzymatic activity can be seen in its amino acid sequence and the environment of the solution it is mixed into. Enzymes are tertiary proteins. The acid can effect the structure by making it less accessible to the substrates or ligands.
What is the role of citric acid in fruit juice concentrate?
add taste,flavour,decrease microbial activity,decrease enzymatic activity,act as antioxidant
