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How are RFLP made?
A DNA sample is broken into pieces by restriction enzymes and the resulting fragments are separated according to their lengths by gel electrophoresis. RFLP analysis was the first DNA profiling technique inexpensive enough to see widespread application. But isn't as widely used now.
What is difference between Bac arrays and DNA arrays?
BAC (Bacterial Artificial Chromosome) arrays are a type of DNA arrays. BAC arrays are usually used for a technique called array CGH (Comparative Genomic Hybridisation) which is used to identify gross deletions or amplifications in DNA (which for example is common in cancer). DNA arrays include BAC arrays but also oligo, cDNA, and promoter arrays. Oligo and cDNA arrays are typically used for gene expression analysis (looking to see how heavily expressed each gene is). Oligo arrays can also be used for SNP (single nucleotide polymorphism) analysis. Promoter arrays are used to identify transcription factor binding sites.
Why is staining the DNA an important step?
Staining DNA is important because it helps visualize the DNA molecules under a microscope or gel electrophoresis. Different stains can highlight different aspects of the DNA, such as the presence of specific sequences or the size of DNA fragments. This allows researchers to analyze and study DNA samples effectively.
In dideoxy sequencing of DNA isn't it so that in each test tube containing a separate ddNTP apart from getting fragments ending at ddNTPs you shall also get the full fragments in each case?
http://wiki.answers.com/Q/In_dideoxy_sequencing_of_dna_isn%27t_it_so_that_in_each_test_tube_containing_a_separate_ddNTP_apart_from_getting_fragments_ending_at_ddNTPs_you_shall_also_get_the_full_fragments_in_each_case" "http://wiki.answers.com/Q/In_dideoxy_sequencing_of_dna_isn%27t_it_so_that_in_each_test_tube_containing_a_separate_ddNTP_apart_from_getting_fragments_ending_at_ddNTPs_you_shall_also_get_the_full_fragments_in_each_case"
What can one do with a DNA kit?
With a DNA kit you can use the swabs and a sample of your saliva to see if someone's DNA matches another person. This is often used to determine paternity of a child.
How are RFLP made?
A DNA sample is broken into pieces by restriction enzymes and the resulting fragments are separated according to their lengths by gel electrophoresis. RFLP analysis was the first DNA profiling technique inexpensive enough to see widespread application. But isn't as widely used now.
If you have a restriction enzyme that cuts a piece of DNA at two recognition sites how many DNA fragments would you see on a gel?
Three.To see why, cut a piece of string in two places! Of course, strictly you would not be able to see only three fragments. You would amplify the DNA before carrying out electrophoresis. That way, you would get perhaps 200 million copies of each fragment, and they would show up. Also, you would only be able to distinguish the fragments if they were different lengths. Electrophoresis separates pieces of DNA by length.
How is gel electrophoresis used in a paternity test to determine biological relationships?
Gel electrophoresis is used in a paternity test to compare the DNA of a child with that of a potential father. By separating the DNA fragments based on size, scientists can see if the child's DNA matches the father's DNA. This helps determine biological relationships with a high level of accuracy.
What is difference between Bac arrays and DNA arrays?
BAC (Bacterial Artificial Chromosome) arrays are a type of DNA arrays. BAC arrays are usually used for a technique called array CGH (Comparative Genomic Hybridisation) which is used to identify gross deletions or amplifications in DNA (which for example is common in cancer). DNA arrays include BAC arrays but also oligo, cDNA, and promoter arrays. Oligo and cDNA arrays are typically used for gene expression analysis (looking to see how heavily expressed each gene is). Oligo arrays can also be used for SNP (single nucleotide polymorphism) analysis. Promoter arrays are used to identify transcription factor binding sites.
Why is gel electrophoresis important?
To separate and analyze DNA fragments and protein fragments by weight. If you have digested some bacterial DNA, for instance, then you can tell by running the fragmented DNA in the gel whether you have digested the correct base length.
What kind of microscope did Watson and crick use to see the DNA molecule?
Watson and Crick used an X-ray diffraction image taken by Rosalind Franklin using a technique primarily developed by Maurice Wilkins. They did not use a microscope to directly visualize the DNA molecule.
Based on restriction maps of plasmid determine the number of DNA fragments and sizes of the fragments?
Plasmids are circular pieces of DNA, so the number of fragments equals the number of cuts from the restriction enzymes. You can easily see this if you start with one restriction enzyme that cuts the plasmid in only one place. Cutting the circle in one place yields you only one fragment. If the restriction cuts in two places, you end up with two fragments; with three places, three fragments, etc. With linear chromosomes, the situation is different. Cutting a linear chromosome in one place yields two fragments, cutting in two places yields three fragments, etc. So the number of fragments is always one more than the number of cuts. A restriction map of a plasmid will show all of the cuts the restriction enzymes made. Each cut is labeled with the enzyme that made it. One can count the spaces between cuts to determine the number of fragments that are produced. Restriction maps usually (but not always) also show the size of each fragment.
Why is staining the DNA an important step?
Staining DNA is important because it helps visualize the DNA molecules under a microscope or gel electrophoresis. Different stains can highlight different aspects of the DNA, such as the presence of specific sequences or the size of DNA fragments. This allows researchers to analyze and study DNA samples effectively.
The results of gel electrophoresis are shown below. What can you determine about the DNA from looking at the results of this test?
Strand 4 is the largest-Apex
In dideoxy sequencing of DNA isn't it so that in each test tube containing a separate ddNTP apart from getting fragments ending at ddNTPs you shall also get the full fragments in each case?
http://wiki.answers.com/Q/In_dideoxy_sequencing_of_dna_isn%27t_it_so_that_in_each_test_tube_containing_a_separate_ddNTP_apart_from_getting_fragments_ending_at_ddNTPs_you_shall_also_get_the_full_fragments_in_each_case" "http://wiki.answers.com/Q/In_dideoxy_sequencing_of_dna_isn%27t_it_so_that_in_each_test_tube_containing_a_separate_ddNTP_apart_from_getting_fragments_ending_at_ddNTPs_you_shall_also_get_the_full_fragments_in_each_case"
Why taping the DNA onto large paper?
Taping DNA onto large paper, also known as DNA FISH mapping, is a technique used to physically stretch out and visualize DNA strands for genetic analysis. It allows researchers to see the genetic information in a linear format, making it easier to study gene sequences, rearrangements, and other structural changes in the DNA. This method is especially useful in cytogenetics to understand the organization of genes along chromosomes.
How does DNA fingerprinting work?
since every one has there own unique DNA, the police can then get samples of it and match that DNA with a record of all other Dana's and see if it matches with one . So they are technically match making.
