Bacteria possess extra chromosomal DNA,called plasmids. Often it carries functional genes for the resistance of bacteria (example: Aromotic compound degrading genes). Plasmid curing is a process of completely removing plasmids of bacteria by means of chemical agents such as Acriflavin or acridine orange!
If the plasmid were cut at more than one site, it could result in the fragmenting of the plasmid into smaller pieces. This could lead to difficulties in maintaining the integrity of the plasmid during cloning processes, affecting the stability and functionality of the plasmid. Additionally, it may disrupt the insertion of foreign DNA or hinder the replication of the plasmid in host cells.
If a plasmid is cut at more than one site by restriction enzymes, it would result in multiple DNA fragments. These fragments can be ligated back together in different combinations, resulting in plasmids with different sizes or configurations. This can lead to the creation of recombinant plasmids with altered properties compared to the original plasmid.
Thriller
Ti plasmid functions to induce turmor or a desease known as "crown gall" to the most dicot (rarely monocot) plants. Transfer DNA or T-DNA will be released during the infection process into the plant cell and integrate with the DNA host. Hence, the plant host is already infected. That's the important function of the Ti plasmid, if there are no such plasmid exist, then the agrobacterium lost its pathogenic function.
Bacteria possess extra chromosomal DNA,called plasmids. Often it carries functional genes for the resistance of bacteria (example: Aromotic compound degrading genes). Plasmid curing is a process of completely removing plasmids of bacteria by means of chemical agents such as Acriflavin or acridine orange!
It would become fragments of DNA and no more the plasmid will be in circular form.
If the plasmid were cut at more than one site, it could result in the fragmenting of the plasmid into smaller pieces. This could lead to difficulties in maintaining the integrity of the plasmid during cloning processes, affecting the stability and functionality of the plasmid. Additionally, it may disrupt the insertion of foreign DNA or hinder the replication of the plasmid in host cells.
If there is a EcoR1 site in either the middle of the Glo gene, or in the middle of the selectable marker site in the plasmid, it would likely disable either Glo, or the plasmid.
Bacterial infection.
bacterial infection
If a plasmid is cut at more than one site by restriction enzymes, it would result in multiple DNA fragments. These fragments can be ligated back together in different combinations, resulting in plasmids with different sizes or configurations. This can lead to the creation of recombinant plasmids with altered properties compared to the original plasmid.
Thriller
Ti plasmid functions to induce turmor or a desease known as "crown gall" to the most dicot (rarely monocot) plants. Transfer DNA or T-DNA will be released during the infection process into the plant cell and integrate with the DNA host. Hence, the plant host is already infected. That's the important function of the Ti plasmid, if there are no such plasmid exist, then the agrobacterium lost its pathogenic function.
Water leaves the cell, causing the cell to shrink.
water leaves the cell causeing the cell to shrink.
It should contain what you think will happen (your hypothesis) and why you think this (to back up your belief). Hope this helps!