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Are restriction endonucleases obtained from various species of bacteria?
Yes
What cuts bacterial plasmids?
Enzymes called restriction endonucleases can cut plasmids. However, in order for a cut to be produced, the plasmid should contain a specific sequence of nucleotides called the restriction site
What is the cutting of DNA by specific enzymes known as restriction endonucleases the cut piece from one organism is added to the DNA of another?
GE
What do you use to cut DNA strands for DNA fingerprints?
A Retsriction enzyme endonuclease is an enzyme that is used to cut DNA strands (both single and double strands) during finger printing at the DNA recognition sites known as restriction sites.
What do biotechnologist used to cut DNA molecules at specific sequences?
Biotechnologists use enzymes called restriction enzymes to cut DNA molecules at specific sequences. These enzymes recognize particular DNA sequences and cut the DNA at those specific locations, allowing for precise manipulation of genetic material.
What is the difference between Restriction endonucleases and exonucleases?
Exonuclease enzymes cleave nucleotides from the ends of DNA molecules. Endonuclease enzymes cleave a phosphodiester bond somewhere within the DNA molecule (not at the ends).
Explain the Molecular mechanism functions of restriction endonucleases?
Restriction Endonucleases recognize certain sites on the DNA or the sequences. For example EcoR1 that recognizes the restriction site GAATTC on any strand of DNA or RNA.
Are restriction endonucleases obtained from various species of bacteria?
Yes
What do biotechnologists use to DNA molecules at specific sequences?
Restriction endonucleases or enzymes
What are the differences between endonucleases and restriction enzymes in terms of their functions and mechanisms of action?
Endonucleases are enzymes that cut DNA at specific sites, while restriction enzymes are a type of endonuclease that specifically recognize and cut DNA at specific sequences called restriction sites. Endonucleases can have various functions in DNA repair and replication, while restriction enzymes are primarily used by bacteria as a defense mechanism against foreign DNA. Both enzymes work by breaking the phosphodiester bonds in the DNA backbone, but restriction enzymes have a more specific recognition and cutting mechanism compared to other endonucleases.
What is meant by the term restriction?
Its the process of cutting DNA molecules into smaller pieces with special enzymes called Restriction Endonucleases (sometimes just called Restriction Enzymes or RE's).
What is the difference between a restriction enzyme and an endonuclease?
A restriction enzyme is a type of endonuclease. Endonucleases are enzymes that cut DNA at specific sequences, while restriction enzymes specifically cut DNA at recognition sites called restriction sites.
What cuts bacterial plasmids?
Enzymes called restriction endonucleases can cut plasmids. However, in order for a cut to be produced, the plasmid should contain a specific sequence of nucleotides called the restriction site
What is the cutting of DNA by specific enzymes known as restriction endonucleases the cut piece from one organism is added to the DNA of another?
GE
What has the author Rebecca Clare Price written?
Rebecca Clare Price has written: 'The effects of restriction endonucleases on mammalian cells of different radiosensitivity'
What chemicals are used to cut DNA into fragments?
Restriction enzymes, also known as restriction endonucleases, are used to cut DNA into fragments by recognizing specific DNA sequences and cleaving the phosphate backbone at these sites. These enzymes are crucial in molecular biology for techniques such as DNA cloning, gene editing, and DNA fingerprinting.
What do you use to cut DNA strands for DNA fingerprints?
A Retsriction enzyme endonuclease is an enzyme that is used to cut DNA strands (both single and double strands) during finger printing at the DNA recognition sites known as restriction sites.
