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The chemicals used to cut DNA fragments are restriction endonucleases, eg- Eco R I, Eco R II,etc
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∙ 11y ago
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∙ 8y agoRestrictive Enzymes
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How are the cleaved DNA fragments from two sources able to recombine?
Sometimes, when the cleaved DNA fragments both have sticky ends, they naturally anneal due to complementary base pairing. However, an enzyme called DNA Ligase is used for joining cut strands of DNA together. There is a ligase called t4 Ligase that joins blunt ends because it is stronger.
Can DNA sequence be cut into smaller fragments by gel electrophoresis?
electrophoresis takes segments of DNA that are already broken up and aligns them by length with an electric current. It doesn't cut the DNA.Added:No, they must be cut into smaller pieces by restriction enzymes ( HINDI, for instance ) before they are run in the gel.
what substance was needed to join the insulin gene to the bacterial DNA?
Technically it is not a substance, but the DNA itself. Let me explain. When the insulin gene is cut out of a regular strand, it is done through a DNA ligase (a cutting enzyme). The SAME ligase is used to cut the bacterial loop of DNA. When ligase cuts DNA sticky ends are left. These sticky ends are, as they say, sticky, and will readily join to new bases with the corresponding (complementary) base pair sequence. As the same ligase is used, the corresponding base sequence is inside the bacterial DNA, so they should connect together.
What do biologists use to cut DNA into smaller fragments?
A restriction enzyme (or restriction endonuclease) is an enzyme that cuts double-stranded or single stranded DNA at specific recognition nucleotide sequences called restriction sites.
What is RFLP used for?
RFLP (often pronounced "rif lip", as if it were a word) is a method used by molecular biologists to follow a particular sequence of DNA as it is passed on to other cells. RFLPs can be used in many different settings to accomplish different objectives. RFLPs can be used in paternity cases or criminal cases to determine the source of a DNA sample. RFLPs can be used determine the disease status of an individual. http://www.bio.davidson.edu/COURSES/genomics/method/RFLP.html
What cuts DNA into fragments?
cutting of DNA into fragments simply means application of suitable restriction enzyme to it.now a days two types of restriction enzymes are available,1)exonucleases,which cut at end portion of DNA and 2)endonucleases ,which cut at specific inner site.
What is used to separate DNA fragments by size?
Electrophoresis. Restriction enzymes are used to cut DNA into fragments. Solutions containing these fragments are placed on the surface of a gel to which an electric current is applied. The electric current causes the DNA fragments to move through the gel. Because smaller fragments move more quickly than larger ones, this process separates the fragments according to size.
After DNA is cut with a restriction enzymes how is the mixture of DNA fragments sorted?
Through the process of gel electrophoresis.
Are restriction enzymes used in gel electrophoresis?
For DNA gel electrophoresis, yes. Once the DNA is cut up into different-sized fragments, they can be electrophoresed to separate bands.
What does the charge of DNA have to do with DNA fingerprinting?
DNA is of a negative charge. So when gel electrophoresis is used on it the DNA fragments are attracted to the positive end of the electrophoresis. The fragments of different lengths travel down the gel towards this end. The longer length fragments travel less and so are farther from the positive end. By looking at these DNA fragments, which are created by cutting DNA with restriction enzymes one can compare and contrast DNA. Thus DNA fingerprinting can take place based on the different restriction sites in DNA (cut by the enzymes) forming different length segments of DNA.
What process is used to cut DNA into fragments?
DNA can be fragmented using restriction endonucleases or restriction enzymes. Restriction enzymes identify specific sequences within the DNA and cause cleavage generating fragments. When this digested DNA is allowed to run in gel electrophoresis fragments get separated according to their mass. When visualized under UV transilluminator, fragmented DNA can be observed as fluorescing bands.
How many lambda DNA fragments does BamHI make?
There are five BamHI cut sites in lambda DNA: Location 5505 22346 27972 34499 41732 So, BamHI will digest lambda DNA into six fragments.
How are the cleaved DNA fragments from two sources able to recombine?
Sometimes, when the cleaved DNA fragments both have sticky ends, they naturally anneal due to complementary base pairing. However, an enzyme called DNA Ligase is used for joining cut strands of DNA together. There is a ligase called t4 Ligase that joins blunt ends because it is stronger.
Can DNA sequence be cut into smaller fragments by gel electrophoresis?
electrophoresis takes segments of DNA that are already broken up and aligns them by length with an electric current. It doesn't cut the DNA.Added:No, they must be cut into smaller pieces by restriction enzymes ( HINDI, for instance ) before they are run in the gel.
If you took a linear piece of DNA and cut it with the restriction enzyme EcoRI and it had three restriction sites for EcoRI, how many fragments would you produce What if you had a circular piece of DNA?
If the plasmid has 3 recognition sequences for a given restriction endonuclease, then 4 linear DNA fragments are obtained because, if the DNA is linear then the number of fragments obtained is (N+1) whereas if the DNA is circular then the number of fragments obtained will be N for N recognition sequences for the given restriction endonuclease in a plasmid.
what substance was needed to join the insulin gene to the bacterial DNA?
Technically it is not a substance, but the DNA itself. Let me explain. When the insulin gene is cut out of a regular strand, it is done through a DNA ligase (a cutting enzyme). The SAME ligase is used to cut the bacterial loop of DNA. When ligase cuts DNA sticky ends are left. These sticky ends are, as they say, sticky, and will readily join to new bases with the corresponding (complementary) base pair sequence. As the same ligase is used, the corresponding base sequence is inside the bacterial DNA, so they should connect together.
What do biologists use to cut DNA into smaller fragments?
A restriction enzyme (or restriction endonuclease) is an enzyme that cuts double-stranded or single stranded DNA at specific recognition nucleotide sequences called restriction sites.
