0
Can DNA sequence be cut into smaller fragments by gel electrophoresis?
Wiki User
∙ 11y ago
electrophoresis takes segments of DNA that are already broken up and aligns them by length with an electric current. It doesn't cut the DNA.
Added:
No, they must be cut into smaller pieces by restriction enzymes ( HINDI, for instance ) before they are run in the gel.
Wiki User
∙ 11y ago
Wiki User
∙ 12y agoGel electrophoresis is used to separate RFLP's of DNA for sequencing .
Add your answer:
Earn +20 pts
DNA fragments can be separated and analyzed by?
Pulse field gel electrophoresis is used to separate DNA fragments by their size.
How do you interpret electrophoresis?
In electrophoresis, DNA is subjected to an electric field which causes the genetic material to migrate in a direction from a cathode to an anode. The DNA that is closest to the anode is determined to be shorter in length compared to the DNA that is closer to the anode. This is explained by fact that the smaller fragments of DNA are better able to travel through the porous gel.
Does gel electrophoresis seperate dna fragments?
dna fragments are negatively charged is the answer for apex.
Explain how an agarose gel can separate DNA fragments of different lengths.?
The separation of DNA fragments is based on size. When a DNA sample is run in a gel (electrophoresis), the lighter fragments migrate faster than the heavier (longer) fragments under the influence of an electric current. At the and of the process, the shorter fragments are found at the terminal end of the gel and the longer fragments closer to the origin
What is maxam Gilbert method of DNA packaging?
Used in DNA sequencing; four samples of end-labeled DNA restriction fragments are chemically cleaved at different specific nucleotides. The resulting subfragments are separated by gel electrophoresis, and the labeled fragments are detected by autoradiography. The sequence of the original end-labeled restriction fragment can be determined directly from parallel electropherograms of the four samples
What is used to separate DNA fragments by size?
Electrophoresis. Restriction enzymes are used to cut DNA into fragments. Solutions containing these fragments are placed on the surface of a gel to which an electric current is applied. The electric current causes the DNA fragments to move through the gel. Because smaller fragments move more quickly than larger ones, this process separates the fragments according to size.
Where do you get the DNA fragments for Gel electrophoresis?
The DNA fragments comes from the method of DNA isolation.
Are the DNA fragments in band 4 larger or smaller than those of band 1?
The size of DNA fragments in band 4 should be smaller than those of band 1. The fragments can be separated by electrophoresis, with the smaller fragments migrating farther than the larger ones.
How are DNA fragments separated on DNA fingerprinting?
Gel electrophoresis
How DNA fragments are sorted by sized?
Electrophoresis
What is a technique for sorting DNA fragments by length?
it is called " electrophoresis"
What are 3 charcteristics that allow gel electrophoresis to work?
The following allow gel electrophoresis to workDifferent sized DNA fragmentsThe migration of DNA under the influence of an electric field in the gel toward the positive electrodeThe different speeds of migration of the DNA fragments - larger fragments moving slower than smaller fragments
How does DNA and gel electrophoresis relate?
Gel electrophoresis separates an individual's DNA fragments from one another according to size. An electric current repels a mixture of the negatively-charged DNA fragments through microscopic pores in the gel from the negative to the positive electrode. Upon completion, the separated fragments of DNA can be visualized as a ladder of small bands in the gel by staining with a methylene blue dye solution or smaller DNA segments move more easily through the gel.
Process that restriction fragments of DNA are separated from each other by the use of electricity?
The process is referred to as gel electrophoresis. This is an analytical process where DNA fragments can be separated based on size within a gel under the influence of an electric field
DNA fragments can be separated and analyzed by?
Pulse field gel electrophoresis is used to separate DNA fragments by their size.
What are the scientific uses for polyacrylamide?
it is used in gel electrophoresis.....for the separation of DNA fragments
After DNA is cut with a restriction enzymes how is the mixture of DNA fragments sorted?
Through the process of gel electrophoresis.
