0
What else can I help you with?
When would you need to use the oil immersion lens?
Not only does oil immersion increase the microscopic resolution of a specimen, it is also transparent. This allows for optimal microscopy views.
What would you observe if you forgot to use oil with the oil immersion lens?
Immersion oil is only required when using a light manuscript at 1000 times magnification (10x from occular lens and 100x from objective lens). At 1000x magnification the image will have poor resolution (loook fuzzy) without the use of immersion oil. This is because the cover slip on the sample and air have different refractory indexes. The light scatter that occurs during the transition from glass to air is noticable at such a high magnification. Immersion oil has a refractive index very similar to the cover slip, thus reducing the light scatter as the light passes from the sample to the objective lens.
Why should you only use the fine adjustment when the high power objective is in position?
Because if you use the coarse adjustment under high power, there is a greater risk that you will crack the slide on the microscope and damage it. The fine adjustment only moves the objective lense small amounts and very slowly, so there is no chance that you will damage the slide.
Which objective should the coarse focus knob be used?
The coarse adjustment knob is only used with the low-power objective lens (usually 4x) because all the other ones are too long and the coarse adjustment knob may bring the slide up too qucikly, therefore it might break the slide or crush the specimen being observed. When using the medium (10x) and high (40x) power objective lens, it is best to use the fine adjustment knob to focus on the details instead.
What must you do if you want to change the objective lens though which you are viewing a specimen?
To change the objective lens when viewing a specimen, you need to rotate the nosepiece of the microscope to select the desired magnification level. Make sure to only adjust the lens by gripping it at its base to prevent damage. Finally, refocus the image using the fine adjustment knob for a clear view.
When would you need to use the oil immersion lens?
Not only does oil immersion increase the microscopic resolution of a specimen, it is also transparent. This allows for optimal microscopy views.
What would you observe if you forgot to use oil with the oil immersion lens?
Immersion oil is only required when using a light manuscript at 1000 times magnification (10x from occular lens and 100x from objective lens). At 1000x magnification the image will have poor resolution (loook fuzzy) without the use of immersion oil. This is because the cover slip on the sample and air have different refractory indexes. The light scatter that occurs during the transition from glass to air is noticable at such a high magnification. Immersion oil has a refractive index very similar to the cover slip, thus reducing the light scatter as the light passes from the sample to the objective lens.
Why should you only use the fine adjustment knob when you are using high power?
The fine adjustment knob should be used with high power magnification because it allows for smaller, more precise movements of the objective lens. This helps to prevent damage to the slide and objective lens, as well as minimize the risk of crashing the objective lens into the slide.
Why is it important to only use the fine adjustment knob when using the highest power objective lens?
When using high power, the lens is very close to the sample - and so you risk damaging the slide and/or lens if they touch - therefore you only want to move the platform a little bit at a time. It is very similar with an oil immersion - you don't want to damage the lens by allowing the sample to touch it.
Why should YOU Only use THE fine adjust when the high power objective IS in positions?
So you don't crack the slide or damage the lens
Why should you use the fine focus knob only when working with the 40x objective lens?
The fine focus knob should be used only with the 40x objective lens because this lens has a narrow depth of field which requires precise adjustments to bring the specimen into focus. Using the fine focus knob with other objective lenses may cause damage to the lens or slide due to the rapid movement of the focusing mechanism.
What must NEVER be used when using the medium or high power objectives?
Oil immersion should never be used with medium or high power objectives. Oil is only used with oil immersion objectives to maximize resolution by reducing light refraction. Using oil with medium or high power objectives could damage the lenses and affect image quality.
Why should the fine adjustment knob be used only with the HPO?
The fine adjustment knob should be used only with the high-power objective (HPO) because it allows for precise focusing at high magnifications. Using the fine adjustment knob with lower-power objectives can result in the objective lens hitting the slide, potentially damaging both the lens and the slide.
Why should you only use the fine adjustment when the high power objective is in position?
Because if you use the coarse adjustment under high power, there is a greater risk that you will crack the slide on the microscope and damage it. The fine adjustment only moves the objective lense small amounts and very slowly, so there is no chance that you will damage the slide.
Why should lens paper only be used once?
lens paper is fragile
How can you tell which objective lens is in the viewing position?
You can typically tell which objective lens is in the viewing position by looking at the number or color marked on the lens. The objectives are usually labeled with magnification values (such as 4x, 10x, 40x, etc.) or distinct colors to help identify them while using a microscope.
How was the compound microscope different from leeuwenhoeks?
Compound microscopes have more than one lens. The first (objective) lens magnifies the object to produce an image. Subsequent lenses magnify an image produced by a previous lens. Leeuwenhoek's "microscope" had only one lens -- simple magnification only. ~The Undertaker~
