According to my bio book, I think it's Alfred Hershey and Martha Chase, in the HERSHEY-CHASE experiment.
Alfred Hershey and Martha Chase conducted the famous Hershey-Chase experiment in 1952, which confirmed that DNA, not protein, is the genetic material in bacteriophages. They used radioactive labeling to trace the genetic material within the bacteriophages, providing evidence for the role of DNA in heredity.
Hershey and Chase discovered that after the P-labeled phages infected the bacteria, most of the radioactive phosphorus was found in the viral DNA, while little to no radioactive sulfur (used to label protein) was found. This confirmed that genetic material, not protein, is responsible for heredity in viruses.
The experiment by Alfred Hershey and Martha Chase in 1952 provided evidence that protein was not the genetic material. They used bacteriophages, which are viruses that infect bacteria, and labeled the DNA with radioactive phosphorus and the protein coat with radioactive sulfur. After allowing the viruses to infect bacteria, they found that only the radioactive phosphorus (DNA) entered the bacterial cells, while the radioactive sulfur (protein) remained outside. This demonstrated that DNA, not protein, was the hereditary material responsible for passing genetic information.
The Hershey-Chase blender experiment helped prove that DNA, not protein, is the genetic material of a bacteriophage. By using radioactive labeling, they were able to track the transmission of genetic material from the virus to the infected bacteria, showing that it was the DNA and not the protein coat that carried the genetic information.
Hershey and Chase attached radioactive sulfur to proteins in the bacteriophage's outer coat in one experiment, and to DNA in another experiment, to determine which molecule was injected into the host bacteria during viral infection. They found that only DNA, not proteins, was passed on to the host bacterium. This confirmed that DNA is the genetic material responsible for heredity.
At the end of Experiment One in the Hershey-Chase experiment, radioactive sulfur, which labeled the protein coats of the T2 bacteriophage, was found in the supernatant, indicating that the protein did not enter the bacterial cells. This suggested that proteins were not the genetic material responsible for transferring traits to the bacteria. In contrast, the radioactive phosphorus used to label DNA was found in the bacterial cells, supporting the conclusion that DNA is the genetic material.
Hershey and Chase observed that DNA is the identity of the hereditary material in phage T2. Hershey and Chase used radioactive labels to mark the protein of the bacteriaphage in one experiment and the DNA in another.
Hershey and Chase observed that DNA is the identity of the hereditary material in phage T2. Hershey and Chase used radioactive labels to mark the protein of the bacteriaphage in one experiment and the DNA in another.
Alfred Hershey and Martha Chase conducted the famous Hershey-Chase experiment in 1952, which confirmed that DNA, not protein, is the genetic material in bacteriophages. They used radioactive labeling to trace the genetic material within the bacteriophages, providing evidence for the role of DNA in heredity.
Hershey and chase
Hershey and Chase discovered that after the P-labeled phages infected the bacteria, most of the radioactive phosphorus was found in the viral DNA, while little to no radioactive sulfur (used to label protein) was found. This confirmed that genetic material, not protein, is responsible for heredity in viruses.
The experiment by Alfred Hershey and Martha Chase in 1952 provided evidence that protein was not the genetic material. They used bacteriophages, which are viruses that infect bacteria, and labeled the DNA with radioactive phosphorus and the protein coat with radioactive sulfur. After allowing the viruses to infect bacteria, they found that only the radioactive phosphorus (DNA) entered the bacterial cells, while the radioactive sulfur (protein) remained outside. This demonstrated that DNA, not protein, was the hereditary material responsible for passing genetic information.
The Hershey-Chase blender experiment helped prove that DNA, not protein, is the genetic material of a bacteriophage. By using radioactive labeling, they were able to track the transmission of genetic material from the virus to the infected bacteria, showing that it was the DNA and not the protein coat that carried the genetic information.
DNA is the genetic material. However, Hershey-Chase also stated that some little sulfur containing material also entered the bacterial cell. Looking at these two conclusions, Hershey-Chase could not reach a definite conclusion as to whether DNA or Protein contains the genetic material. The conclusion came from Watson-Crick who stated that the sulfur containing material could have entered the cell due to the fact that DNA replication and subsequently translation of the mRNA leads to the synthesis of sulfur containing protein which were found in the bacterial cell.
Their genetic material
Their genetic material
Hershey and Chase attached radioactive sulfur to proteins in the bacteriophage's outer coat in one experiment, and to DNA in another experiment, to determine which molecule was injected into the host bacteria during viral infection. They found that only DNA, not proteins, was passed on to the host bacterium. This confirmed that DNA is the genetic material responsible for heredity.