bunny versus pie
Hershey and Chase used radioactive sulfur-35 to tag phage proteins and radioactive phosphorus-32 to tag phage DNA in their experiments on bacteriophages.
Hershey and Chase used radioactive Sulfur to label viral proteins, as proteins contain sulfur. By growing the viruses in a culture containing both radioactive Sulfur and Phosphorus, they could differentiate between viral proteins (labeled with Sulfur) and viral DNA (labeled with Phosphorus). If they had only used one radioactive substance, they would not have been able to determine the specific molecule (protein or DNA) that the virus injected into the host cell.
Hershey and Chase discovered that after the P-labeled phages infected the bacteria, most of the radioactive phosphorus was found in the viral DNA, while little to no radioactive sulfur (used to label protein) was found. This confirmed that genetic material, not protein, is responsible for heredity in viruses.
According to my bio book, I think it's Alfred Hershey and Martha Chase, in the HERSHEY-CHASE experiment.
The experiment by Alfred Hershey and Martha Chase in 1952 provided evidence that protein was not the genetic material. They used bacteriophages, which are viruses that infect bacteria, and labeled the DNA with radioactive phosphorus and the protein coat with radioactive sulfur. After allowing the viruses to infect bacteria, they found that only the radioactive phosphorus (DNA) entered the bacterial cells, while the radioactive sulfur (protein) remained outside. This demonstrated that DNA, not protein, was the hereditary material responsible for passing genetic information.
Hershey and Chase used radioactive sulfur-35 to tag phage proteins and radioactive phosphorus-32 to tag phage DNA in their experiments on bacteriophages.
At the end of Experiment One in the Hershey-Chase experiment, radioactive sulfur, which labeled the protein coats of the T2 bacteriophage, was found in the supernatant, indicating that the protein did not enter the bacterial cells. This suggested that proteins were not the genetic material responsible for transferring traits to the bacteria. In contrast, the radioactive phosphorus used to label DNA was found in the bacterial cells, supporting the conclusion that DNA is the genetic material.
Hershey and Chase used radioactive Sulfur to label viral proteins, as proteins contain sulfur. By growing the viruses in a culture containing both radioactive Sulfur and Phosphorus, they could differentiate between viral proteins (labeled with Sulfur) and viral DNA (labeled with Phosphorus). If they had only used one radioactive substance, they would not have been able to determine the specific molecule (protein or DNA) that the virus injected into the host cell.
Hershey and Chase
Hershey and chase
Hershey and Chase discovered that after the P-labeled phages infected the bacteria, most of the radioactive phosphorus was found in the viral DNA, while little to no radioactive sulfur (used to label protein) was found. This confirmed that genetic material, not protein, is responsible for heredity in viruses.
According to my bio book, I think it's Alfred Hershey and Martha Chase, in the HERSHEY-CHASE experiment.
Hershey and Chase observed that DNA is the identity of the hereditary material in phage T2. Hershey and Chase used radioactive labels to mark the protein of the bacteriaphage in one experiment and the DNA in another.
Hershey and Chase observed that DNA is the identity of the hereditary material in phage T2. Hershey and Chase used radioactive labels to mark the protein of the bacteriaphage in one experiment and the DNA in another.
Phosphorous 32 was used to label the Nucleic Acid. Sulfur 35 was used to label the protein. The progeny of the E. coli cells Hershey and Chase grew the virus in had Phosphorous 32 in them which concluded that the DNA went inside and was the genetic material. The phage coating left on the outside tested positive for Sulfur 35 which meant protein was left on the outside.
According to my bio book, I think it's Alfred Hershey and Martha Chase, in the HERSHEY-CHASE experiment.
Hershey and Chase's experiment worked because they used radioactive labeling to track the DNA and protein separately. This allowed them to determine that only the DNA, not the protein, was passed on to the next generation of bacteria.