Hershey and Chase used radioactive sulfur-35 to tag phage proteins and radioactive phosphorus-32 to tag phage DNA in their experiments on bacteriophages.
Hershey and Chase used radioactive Sulfur to label viral proteins, as proteins contain sulfur. By growing the viruses in a culture containing both radioactive Sulfur and Phosphorus, they could differentiate between viral proteins (labeled with Sulfur) and viral DNA (labeled with Phosphorus). If they had only used one radioactive substance, they would not have been able to determine the specific molecule (protein or DNA) that the virus injected into the host cell.
Hershey and Chase discovered that after the P-labeled phages infected the bacteria, most of the radioactive phosphorus was found in the viral DNA, while little to no radioactive sulfur (used to label protein) was found. This confirmed that genetic material, not protein, is responsible for heredity in viruses.
These two isotopes are: phosphorus-32 and sulfur-35.
Phosphorous 32 was used to label the Nucleic Acid. Sulfur 35 was used to label the protein. The progeny of the E. coli cells Hershey and Chase grew the virus in had Phosphorous 32 in them which concluded that the DNA went inside and was the genetic material. The phage coating left on the outside tested positive for Sulfur 35 which meant protein was left on the outside.
Hershey and Chase's decision to use radioactive phosphorus and sulfur was appropriate because these elements are found in DNA and protein, respectively, which allowed them to track the transmission of genetic material. Using carbon or oxygen would not have been effective as they are not prevalent in DNA or proteins and would not provide the same specificity in tracking genetic material.
Hershey and Chase attached radioactive sulfur to proteins in the bacteriophage's outer coat in one experiment, and to DNA in another experiment, to determine which molecule was injected into the host bacteria during viral infection. They found that only DNA, not proteins, was passed on to the host bacterium. This confirmed that DNA is the genetic material responsible for heredity.
Hershey and chase
Hershey and Chase
According to my bio book, I think it's Alfred Hershey and Martha Chase, in the HERSHEY-CHASE experiment.
Hershey and Chase observed that DNA is the identity of the hereditary material in phage T2. Hershey and Chase used radioactive labels to mark the protein of the bacteriaphage in one experiment and the DNA in another.
Hershey and Chase observed that DNA is the identity of the hereditary material in phage T2. Hershey and Chase used radioactive labels to mark the protein of the bacteriaphage in one experiment and the DNA in another.