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Phenolphthalein and bromophenol blue are used as indicators due to their distinct color changes at specific pH ranges, making them useful for determining acidity or alkalinity in various solutions. Phenolphthalein transitions from colorless to pink as the pH increases from around 8.2 to 10, while bromophenol blue shifts from yellow to blue between pH 6.0 and 7.6. These properties allow them to effectively signal the endpoint in titrations and other chemical analyses. Their clear visual indicators facilitate easy interpretation of results in laboratory settings.
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What is bromophenol blue used for?
It is used as a pH (acid-base) indicator, particularly for substances with an expected pH between 3.0 and 4.6. Bromophenol blue turns yellow at or below a pH of 3.0. It turns purple at or above a pH of 4.6.
What is the role of bromophenol blue is plasmid DNA isolation?
Bromophenol blue is the tracking dye in electrophoresis. Being of small molecular size, it races towards the other electrode before the DNA. It is used so that you don't mistakenly let the DNA get washed off the gel and into the buffer solution.
What are two commonly used indicators are bromthymol blue and?
Two commonly used indicators are bromothymol blue and phenolphthalein. Bromothymol blue is typically used in acid-base titrations, changing from yellow in acidic solutions to blue in alkaline conditions. Phenolphthalein, on the other hand, is colorless in acidic solutions and turns pink in alkaline environments, making it another popular choice for similar applications. Both indicators help visualize pH changes during chemical reactions.
What is the function of Bromophenol blue and Glycerol when used in electrophoresis?
Bromophenol blue is a tracking dye used in electrophoresis to visualize the progress of sample migration through the gel; it migrates at a rate similar to small proteins, allowing researchers to gauge the separation of samples. Glycerol, on the other hand, increases the density of the sample loading solution, ensuring that the samples sink into the wells of the gel rather than diffusing into the buffer. Together, they facilitate effective sample loading and monitoring during the electrophoresis process.
What are the types of indicators used in titration reaction labs?
Indicator ______________Color change ___________pH at end pointMethyl orange____________orange ---> yellow_______ 3.7Litmus__________________ red ---> blue___________ 6.5Bromothylmol blue ________yelow --->blue___________7Phenolphthalein___________Colorless---> pink________9.1
What are some indicators of acids?
Methyl Red, methyl orange, bromophenol blue, phenolphthalein, thymolphthalein, bromocresol green, bromothymol blue are all used as acid base indicators.
Does Amax of bromophenol blue vary with concentration?
Yes, the absorbance maximum (Amax) of bromophenol blue does vary with concentration. As the concentration of bromophenol blue increases, the Amax shifts from its initial value. This change in Amax can be used to determine the concentration of bromophenol blue in a solution through spectrophotometric analysis.
What can you use in place of bromophenol blue?
Methyl orange or phenolphthalein can be used as alternatives to bromophenol blue in certain experiments requiring a pH indicator. It is important to consider the specific requirements of the experiment and the pH range of the indicator being used.
What is bromophenol blue used for?
It is used as a pH (acid-base) indicator, particularly for substances with an expected pH between 3.0 and 4.6. Bromophenol blue turns yellow at or below a pH of 3.0. It turns purple at or above a pH of 4.6.
Can you use indicator other than phenolphthalein in the titration of NaOH?
Yes, you can use indicators such as bromothymol blue or methyl orange in the titration of NaOH. These indicators change color at different pH ranges and can be used based on the specific pH endpoint needed for the titration.
Why two stains required in SDS PAGE- bromophenol blue and coomassie blue?
Bromophenol blue is a tracking dye used to monitor the progress of electrophoresis, helping visualize the migration of proteins in the gel. Coomassie blue is a protein dye used for staining proteins after electrophoresis, allowing for their visualization and quantification. Both stains serve distinct purposes in the SDS-PAGE process to ensure accurate results and analysis.
What is the use of phenapthalein?
if you mean Phenolphthalein, it is used as a laxative. In the laboratory it is the indicator of the acidity of solutions
What is the role of bromophenol blue is plasmid DNA isolation?
Bromophenol blue is the tracking dye in electrophoresis. Being of small molecular size, it races towards the other electrode before the DNA. It is used so that you don't mistakenly let the DNA get washed off the gel and into the buffer solution.
What is the Composition of gel loading dye used in DNA amplification?
A typical gel loading dye used in DNA amplification consists of tracking dyes (such as bromophenol blue or xylene cyanol FF) to monitor the progress of DNA migration in gel electrophoresis, as well as a densifying agent (such as glycerol) to help the sample sink into the gel wells. Some formulations may also contain a reducing agent (like DTT) to prevent DNA degradation.
What is used to indentifiy bases and acids?
Acid base indicators are used to identify acids and bases. Among the common indicators are;PhenolphthaleinMethyl redMethyl orangeBromothymol blue
What substance is used as an acid-base indicator?
Phenolphthalein, Metyl Orange, Methyl Red, Thymol Blue and Bromothymol Blue are some of the commonly used acid-base indicators. The property of such indicators should be the capability of showing a distinct chemical change with respect to a particular pH value.
What are the types of indicators used in titration reaction labs?
Indicator ______________Color change ___________pH at end pointMethyl orange____________orange ---> yellow_______ 3.7Litmus__________________ red ---> blue___________ 6.5Bromothylmol blue ________yelow --->blue___________7Phenolphthalein___________Colorless---> pink________9.1
