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It is used as a pH (acid-base) indicator, particularly for substances with an expected pH between 3.0 and 4.6.

Bromophenol blue turns yellow at or below a pH of 3.0.

It turns purple at or above a pH of 4.6.

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What is the colour of bromophenol blue when neutral?

Bromophenol blue is green when neutral.


Does Amax of bromophenol blue vary with concentration?

Yes, the absorbance maximum (Amax) of bromophenol blue does vary with concentration. As the concentration of bromophenol blue increases, the Amax shifts from its initial value. This change in Amax can be used to determine the concentration of bromophenol blue in a solution through spectrophotometric analysis.


What can you use in place of bromophenol blue?

Methyl orange or phenolphthalein can be used as alternatives to bromophenol blue in certain experiments requiring a pH indicator. It is important to consider the specific requirements of the experiment and the pH range of the indicator being used.


Why two stains required in SDS PAGE- bromophenol blue and coomassie blue?

Bromophenol blue is a tracking dye used to monitor the progress of electrophoresis, helping visualize the migration of proteins in the gel. Coomassie blue is a protein dye used for staining proteins after electrophoresis, allowing for their visualization and quantification. Both stains serve distinct purposes in the SDS-PAGE process to ensure accurate results and analysis.


What does dye contain?

The loading dye comprises bromophenol blue, Ficoll 400 and water majorly while Xylene cyanol, Tris and EDTA are optional in it. Bromophenol blue is one of the most popular indicators of DNA in agarose gel electrophoresis. Bromophenol blue is a pH indicator.


Which is more polar bromophenol blue or phenolphthalein?

Phenolphthalein is more polar than bromophenol blue due to its structure and functional groups. Phenolphthalein contains more oxygen atoms that can participate in hydrogen bonding, making it a more polar compound compared to bromophenol blue.


What is the pka of bromophenol blue?

Phenols are acidic substances (pH over 7).


What happens if you mix bromphenol blue and NaOH?

When bromophenol blue is mixed with NaOH, the solution turns blue because the pH becomes alkaline. Bromophenol blue is a pH indicator that changes color in different pH ranges. In the presence of NaOH, which is a base, the bromophenol blue changes from yellow (at acidic pH) to blue (at alkaline pH).


Can you use bromothymol blue in place of bromophenol blue?

no.heres why:bromophenol-blue-1bromothymol-blue-1


Can you use bromophenol blue in place of bromothymol blue?

No, bromophenol blue and bromothymol blue are not interchangeable. They have different pH ranges for color changes and may not give accurate results if substituted for each other in experiments or procedures.


If you add a bromophenol blue solution to .01M HCl what solution color would result after mixing?

The bromophenol blue will turn yellow in the presence of an acidic solution like 0.01M HCl.


What is the role of bromophenol blue is plasmid DNA isolation?

Bromophenol blue is the tracking dye in electrophoresis. Being of small molecular size, it races towards the other electrode before the DNA. It is used so that you don't mistakenly let the DNA get washed off the gel and into the buffer solution.