0
Bromophenol blue is a pH indicator that stains proteins, making it useful for visualizing organelles such as the endoplasmic reticulum and Golgi apparatus in cells. These organelles often contain a high concentration of proteins, which can be detected when stained with bromophenol blue. Additionally, the cytoplasm may also exhibit staining due to its protein content. However, it is important to note that bromophenol blue is not specific to any organelle and can stain various cellular components.
What else can I help you with?
What is the pka of bromophenol blue?
Phenols are acidic substances (pH over 7).
Why you put bromophenol blue in lysis buffer?
Bromophenol blue is added to lysis buffer as a tracking dye to monitor the progress of protein electrophoresis. It helps visualize the sample migration through the gel during SDS-PAGE by imparting a blue color to the proteins.
What are the colours of the cell organelles after being stained?
It depends on the type of stain used. H&E stain, stains nuclei and granules blue and other eosinophilic structures as red, pink, or orange. CV stains produce a purple color. Eosin produes red.
What is bromophenol blue used for?
It is used as a pH (acid-base) indicator, particularly for substances with an expected pH between 3.0 and 4.6. Bromophenol blue turns yellow at or below a pH of 3.0. It turns purple at or above a pH of 4.6.
What are some of the most common acid and base indicators?
Phenolphthalein Methyl Orange Litmus Bromophenol Blue
What is the colour of bromophenol blue when neutral?
Bromophenol blue is green when neutral.
Does Amax of bromophenol blue vary with concentration?
Yes, the absorbance maximum (Amax) of bromophenol blue does vary with concentration. As the concentration of bromophenol blue increases, the Amax shifts from its initial value. This change in Amax can be used to determine the concentration of bromophenol blue in a solution through spectrophotometric analysis.
What does dye contain?
The loading dye comprises bromophenol blue, Ficoll 400 and water majorly while Xylene cyanol, Tris and EDTA are optional in it. Bromophenol blue is one of the most popular indicators of DNA in agarose gel electrophoresis. Bromophenol blue is a pH indicator.
Which is more polar bromophenol blue or phenolphthalein?
Phenolphthalein is more polar than bromophenol blue due to its structure and functional groups. Phenolphthalein contains more oxygen atoms that can participate in hydrogen bonding, making it a more polar compound compared to bromophenol blue.
What is the pka of bromophenol blue?
Phenols are acidic substances (pH over 7).
What happens if you mix bromphenol blue and NaOH?
When bromophenol blue is mixed with NaOH, the solution turns blue because the pH becomes alkaline. Bromophenol blue is a pH indicator that changes color in different pH ranges. In the presence of NaOH, which is a base, the bromophenol blue changes from yellow (at acidic pH) to blue (at alkaline pH).
Can you use bromothymol blue in place of bromophenol blue?
no.heres why:bromophenol-blue-1bromothymol-blue-1
Can you use bromophenol blue in place of bromothymol blue?
No, bromophenol blue and bromothymol blue are not interchangeable. They have different pH ranges for color changes and may not give accurate results if substituted for each other in experiments or procedures.
If you add a bromophenol blue solution to .01M HCl what solution color would result after mixing?
The bromophenol blue will turn yellow in the presence of an acidic solution like 0.01M HCl.
Is it useful to stain the Elodea and Anabaena cells with methylene blue stain?
Staining Elodea and Anabaena cells with methylene blue can be useful in microscopy to enhance contrast and visualize cell structures more clearly. Methylene blue is commonly used as a general stain to highlight cellular components such as nuclei and cytoplasm. This staining technique can aid in identifying cellular organelles and structures during microscopic examination.
Why bromophenol blue is blue color?
Bromophenol blue is blue in color because of its molecular structure, which contains a conjugated system of alternating single and double bonds that allows it to absorb light in the visible spectrum, particularly in the blue region. This absorption of blue light gives the compound its characteristic blue color.
Why you put bromophenol blue in lysis buffer?
Bromophenol blue is added to lysis buffer as a tracking dye to monitor the progress of protein electrophoresis. It helps visualize the sample migration through the gel during SDS-PAGE by imparting a blue color to the proteins.
