0
What else can I help you with?
Can you use bromophenol blue in place of bromothymol blue?
No, bromophenol blue and bromothymol blue are not interchangeable. They have different pH ranges for color changes and may not give accurate results if substituted for each other in experiments or procedures.
What can you use in place of bromophenol blue?
Methyl orange or phenolphthalein can be used as alternatives to bromophenol blue in certain experiments requiring a pH indicator. It is important to consider the specific requirements of the experiment and the pH range of the indicator being used.
Can you use indicator other than phenolphthalein in the titration of NaOH?
Yes, you can use indicators such as bromothymol blue or methyl orange in the titration of NaOH. These indicators change color at different pH ranges and can be used based on the specific pH endpoint needed for the titration.
Is bromothymol blue in blue food coloring?
No, bromothymol blue is not typically used in blue food coloring. Blue food coloring usually contains synthetic colorants such as Brilliant Blue FCF (Blue 1) or Indigo Carmine (Blue 2), which are specifically approved for food use by regulatory agencies. Bromothymol blue is more commonly used as a pH indicator in laboratories and not intended for consumption.
How does the pH indicator bromothymol blue measure cellular respiration?
He can use a chemical reaction when it is mixed with CO2
How do I prepare bromophenol indicator solution?
classic recipes say 0.25% bromphenol blue (0.25g/100ml) in a solution containing a viscous substance like: 40%sucrose, or 15%Ficoll, or 30%glycerol all in water. Personally, I use glycerol.
What does photosynthesis have to do with the use of Bromothymol Blue?
Bromothymol blue is an indicator which changes colour in the presence of an acid. When carbon dioxide mixes with water it produces carbonic acid (soda water). A pretty common experiment involves putting a respiring organism in water mixed with bromothymol blue, the water should turn yellow as the organism takes in oxygen and produces carbon dioxide. The indicator can be changed back to its original blue colour by removing the carbon dioxide from the water, i.e. putting in a plant to run photosynthesis - drawing out the carbon dioxide to produce oxygen.
What is a limitation of Bromothymol Blue?
Bromothymol Blue is a liquid indicator that can detect presence CO2 in water. It changes color from blue to green, as it detects the presence of the CO2 . In very acidic conditions it will turn yellow. It has also been used as an indicator in the NCO (isocyanate) test (where di-n-butylamine is the base and 1N HCl solution is the acid. Green is the endpoint in this titration and yellow is "over-shot". The amine value test in resin chemistry/synthesis also may use this indicator. HCl directly titrates an amine containing resin.
What is Bromothymol?
Bromothymol Blue is a liquid indicator that can detect presence CO2 in water. It changes color from blue to green, as it detects the presence of the CO2 . In very acidic conditions it will turn yellow. It has also been used as an indicator in the NCO (isocyanate) test (where di-n-butylamine is the base and 1N HCl solution is the acid. Green is the endpoint in this titration and yellow is "over-shot". The amine value test in resin chemistry/synthesis also may use this indicator. HCl directly titrates an amine containing resin.
Function of bromophenolblue in agarose gel electrophoresis?
Bromophenol blue or commasive blue functions as a sample staining dye or DNA staining dye it is mixed with sample before loading the sample in wells. The migration of bromophenol blue is same as of DNA i.e. it carries negative charge and move in same direction of DNA with the speed equals to 200-400bp of DNA.It also prevent backflow of sample in vertical gel electrophoresis as the sample is light from the loading buffer which tends to come back from the well so bromophenol blue prevent the back flow.IUPAC NAME:2,6-dibromo-4-[3-(3,5-dibromo-4-hydroxyphenyl)-1,1-dioxo-3-benzooxathiolyl]phenol.Bromphenol blue does not stain DNA. It is simply a dye that 1) helps you visualise your sample as you load it and 2) migrates (unrelated to the DNA) at a speed that is indeed equivalent to about 200-400bp of DNA, depending on the percentage of gel, giving an indication of how far your samples have run. It also does not prevent "backflow". Usually the buffer which you add to your DNA sample before loading on a gel (ie loading buffer) contains a dye such as bromophenol blue (there are others) and will also contain a dense substance, usually glycerol or ficoll. It is the glycerol or ficoll which due to its density will make the sample more dense than the buffer which the gel is run in, and will prevent it floating out of the well.In order to visualise (stain) the DNA you need an agent such as ethidium bromide or sybr green that intercalates with the DNA (slides between the basepairs) and fluoresces under UV light.Coommassie (not commasive) blue is a dye that will stain proteins (not DNA) but is used after the gel has been run to stain the gel. If you use it with an agarose gel, I'm guessing - having never tried it) you would just simply make a big blue mess and not see anything.
What eles can you use for phenol red?
Other substances that can be used as pH indicators include bromothymol blue, methyl red, and litmus. These indicators change color at different pH levels, providing a visual indication of the acidity or alkalinity of a solution.
How do you beat level 18 on factory balls 2?
place blue on first, use cap then place red , add blue again .... so on
