0
What else can I help you with?
Small accessory chromosomes found in bacteria and useful in recombinant DNA procedures are called?
plasmids
What does plasmids do?
plasmids are vectors that are used to replicate recombinant DNA in a host organism or cell. they are normally used in cloning experiments.
What would happen if a plasmid was cut at more than one site?
If a plasmid is cut at more than one site by restriction enzymes, it would result in multiple DNA fragments. These fragments can be ligated back together in different combinations, resulting in plasmids with different sizes or configurations. This can lead to the creation of recombinant plasmids with altered properties compared to the original plasmid.
What is recombinant Plasmid?
A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell's chromosomal DNA. ... Researchers can insert DNA fragments or genes into a plasmid vector, creating a so-called recombinant plasmid. This plasmid can be introduced into a bacterium by way of the process called transformation.
What is the difference between the original plasmid and the recombinant plasmid?
Orginal Plasmids are extra chromosomal genetic material present in eukaryotes and some prokaryotes.Recombinant plasmids contain a gene of intrest ie,individual gene carrying a specific function can be inserted in to a specific site on original plasmid in cell culture via transformation.So the recombinant plasmid contain both gene of intrest and native genes.
Small accessory chromosomes found in bacteria and useful in recombinant DNA procedures are called?
plasmids
What is the specific DNA sequence recognized by the EcoRI restriction enzyme, known as the EcoRI cut site?
The specific DNA sequence recognized by the EcoRI restriction enzyme, known as the EcoRI cut site, is 5'-GAATTC-3'.
What does plasmids do?
plasmids are vectors that are used to replicate recombinant DNA in a host organism or cell. they are normally used in cloning experiments.
Where does the restriction enzyme EcoRI cut in a DNA sequence?
The restriction enzyme EcoRI cuts DNA at a specific sequence of bases, which is GAATTC.
Which restriction enzyme did you use to cut the DNA?
The restriction enzyme used to cut the DNA was EcoRI.
Why are plasmids essential for recombinant DNA technology?
Plasmids are essential for recombinant DNA technology because they are small, circular DNA molecules that can be easily manipulated and transferred between different organisms. They serve as vectors to carry foreign DNA into host cells, allowing for the creation of genetically modified organisms.
A collection of cloned recombinant plasmids that includes fragments from the entire genome of a cell is called a?
Genomic library
What would happen if a plasmid was cut at more than one site?
If a plasmid is cut at more than one site by restriction enzymes, it would result in multiple DNA fragments. These fragments can be ligated back together in different combinations, resulting in plasmids with different sizes or configurations. This can lead to the creation of recombinant plasmids with altered properties compared to the original plasmid.
What is recombinant Plasmid?
A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell's chromosomal DNA. ... Researchers can insert DNA fragments or genes into a plasmid vector, creating a so-called recombinant plasmid. This plasmid can be introduced into a bacterium by way of the process called transformation.
What are the steps to Genetically modified organism?
first you have to take the gene of interest (the gene that you want to put into the bacteria) and get it cut with restriction enzymes. then mix it with the plasmids from the bacteria. then you put the mixture, which is now called a recombinant DNA into the bacteria and incubate it so it can grow.
What is recombinant sequence?
A recombinant sequence of DNA is a sequence of DNA that comes from more than one source. Examples of recombinant DNA are plasmids that are put into bacteria. The plasmid comes from the bacteria (or a bacteria at least) but a target gene has been added (say the lac operon gene that allows bacteria to thrive on lactose), this plasmid is now a recombinant DNA sequence.
What is the difference between the original plasmid and the recombinant plasmid?
Orginal Plasmids are extra chromosomal genetic material present in eukaryotes and some prokaryotes.Recombinant plasmids contain a gene of intrest ie,individual gene carrying a specific function can be inserted in to a specific site on original plasmid in cell culture via transformation.So the recombinant plasmid contain both gene of intrest and native genes.
