Well, first of all, the colonies growing on the surface of the agar medium are aerobic . . . that is, they need air - Oxygen - to survive.
The anaerobic colonies growing within the agar medium may simply be slower growing or maturing, for some reason. Perhaps that is simply due to their being anaerobic.
The surface colonies on a pour plate larger than those within the medium especially aerobic bacteria within the medium would be a restriction of growth. The restriction of growth would be due to the lack of oxygen.
That really depends on what agar you are growing them on. If you are using growing agars such as BHI or blood, both will grow, and you won't be able to tell the difference when looking at them on the plate. If you use a selective agar that, lets say, will only grow cocci, then the bacilli won't grow. Or vice versa, if your selective agar only grows bacilli, then the cocci won't grow. If you use a differential agar, it may turn one a different color than the other -- for example, it may turn the bacillus blue, and the cocci may turn red. It is all dependent on the media you choose to grow your samples.
Proving the purity of a culture is generally more rapid on agar than in broth. Agar plates allow for visual inspection of colonies, making it easier to identify and isolate pure cultures quickly. In contrast, broth cultures may require further sub-culturing or streaking onto agar to confirm purity, which can take more time. Overall, the solid medium of agar facilitates faster assessment of purity through colony morphology.
Diffusion is slower in agar than in water because agar is a gelatinous substance that contains solid particles which obstruct the free movement of molecules. This impedes the diffusion of molecules through the agar compared to the unrestricted movement in water, which lacks solid particles.
Gelatin is less suitable than agar as a solidifying agent for bacteriological media primarily because it is not stable at higher temperatures and can liquefy during incubation. Additionally, gelatin can be digested by some bacteria, leading to inconsistent results and difficulties in isolating colonies. Agar, on the other hand, remains solid at a wide range of temperatures and is not metabolized by most microorganisms, making it a more reliable medium for bacterial growth.
With the climate harsh there is a short growing season, there for there are small farms than the middle colonies.
The surface colonies on a pour plate larger than those within the medium especially aerobic bacteria within the medium would be a restriction of growth. The restriction of growth would be due to the lack of oxygen.
Slants are better suited than agar plates, because they can be capped, preventing the agar and the culture from drying out. The cap also prevents airborne contaminants from entering the slant. Also, slants take up less storage space than an agar plate.
Not necessarily, some Bacillus can have big colonies
New England colonies had land that was mainly filled with rocks, or sand.
No the Middle Colonies had bigger farms :)
That really depends on what agar you are growing them on. If you are using growing agars such as BHI or blood, both will grow, and you won't be able to tell the difference when looking at them on the plate. If you use a selective agar that, lets say, will only grow cocci, then the bacilli won't grow. Or vice versa, if your selective agar only grows bacilli, then the cocci won't grow. If you use a differential agar, it may turn one a different color than the other -- for example, it may turn the bacillus blue, and the cocci may turn red. It is all dependent on the media you choose to grow your samples.
New England farms were smaller because with cold climate,poor soil and short growing season to stop agriculture.The Southern Colonies were like the opposite.
No, the Sahara is larger than Brazil. Brazil is about 3.2 million square miles and the Sahara 3.3 and growing.
The southern colonies are more conducive to agriculture than the northern colonies due to their warmer climate, longer growing seasons, and fertile soil. These factors allow for a wider variety of crops to be grown and harvested successfully in the southern colonies compared to the northern colonies.
cause the farms in this fertile area were larger and the people lived farther apart than the people in New England.
Streaking in a straight line on an agar slant helps to isolate individual colonies of bacteria as they grow. A back and forth wavy inoculation could result in overlapping colonies, making it difficult to observe and identify individual colonies. Streaking in a straight line also ensures a uniform distribution of bacteria across the medium.