You can use the coarse adjustment knob with the low-power objective lens because it provides a wider field of view and greater depth of focus, making it easier to bring the specimen into general focus. However, using the coarse adjustment with the high-power objective lens risks crashing the lens into the slide, which can damage both the lens and the specimen. Therefore, fine focusing is necessary at higher magnifications to achieve precise focus without risking damage.
First of all, one should never call it high power, it is morecommonly called the "high objective", yet that is not what this question is asking. To answer the question: You use the fine adjustment knob. This knob should be located near the coarse adjustment knob, on the opposite side of the microscope (at the same height as the coarse adjustment knob), or even as a separate knob protruding from the coarse adjustment knob. The fine adjustment knob is smaller in size. You should never, ever use the coarse adjustment knob under the high objective, you could scratch the microscope slide, cover slip, high objective lens, or in a worst case scenario break the high objective lens.
The depth of field is so small that a very small vertical travel could shift the focus from the top of the specimen to the bottom. A very fine adjustment must be used, which is why the coarse adjustment screw is implemented.
One thing that can happen if you use the coarse adjustment while the oil immersion objective is in place is that the slide will end up breaking. It is better to use the fine adjustment.
The fine adjustment screw is used when focusing the 40X objective. The coarse adjustment screw should only be used with lower magnifications to bring the specimen into rough focus.
Nothing will happen because the coarse adjustment knob controls the position of the stage of the microscope, which is used to focus the specimen. This adjustment is independent of the choice of objective lens.
The coarse adjustment knob moves the objective a lot while the fine adjustment knob moves it just a little, so using the coarse knob under high power can cause the objective to hit the slide and potentially damage the slide or the objective. The fine adjustment knob provides more precise control for focusing under high power, allowing for better clarity and accuracy.
The adjustment knob that moves the body tube for focusing with the high-power objective lens is called the fine focus adjustment knob. It allows for precise adjustments to bring the specimen into sharp focus at high magnifications.
The course adjustment moves the stage of the microscope further/closer away from the objective lens (it is used for general focusing), and the fine adjustment moves the objective lens very small distances for finer focusing.
First of all, one should never call it high power, it is morecommonly called the "high objective", yet that is not what this question is asking. To answer the question: You use the fine adjustment knob. This knob should be located near the coarse adjustment knob, on the opposite side of the microscope (at the same height as the coarse adjustment knob), or even as a separate knob protruding from the coarse adjustment knob. The fine adjustment knob is smaller in size. You should never, ever use the coarse adjustment knob under the high objective, you could scratch the microscope slide, cover slip, high objective lens, or in a worst case scenario break the high objective lens.
The coarse adjustment knob and the fine adjustment knob are used to move the objective lens up and down to focus on the specimen.
Coarse adjustment in a microscope is used to quickly focus on the specimen by moving the stage or objective lens up or down in large increments. This adjustment is used to bring the specimen into view before using the fine adjustment for finer focusing details.
The depth of field is so small that a very small vertical travel could shift the focus from the top of the specimen to the bottom. A very fine adjustment must be used, which is why the coarse adjustment screw is implemented.
eyepiece lens, arm, base, tube, illuminator, objective lenses, diaphragm, and condenser lens
Turning the coarse adjustment knob of a microscope downwards brings the objective lens closer to the specimen, allowing for initial focusing at low magnification. This is useful for quickly bringing the specimen into view. Turning the coarse adjustment knob upwards moves the objective lens away from the specimen, enabling a higher focal point for fine-tuning the focus at higher magnifications. It is essential to use the coarse adjustment first to avoid crashing the objective lens into the specimen, which can damage both the lens and the specimen.
One thing that can happen if you use the coarse adjustment while the oil immersion objective is in place is that the slide will end up breaking. It is better to use the fine adjustment.
The fine adjustment screw is used when focusing the 40X objective. The coarse adjustment screw should only be used with lower magnifications to bring the specimen into rough focus.
For low power you can use the coarse adjustment and fine adjustment for high power you should only use the fine adjustment because on high power, the objective lens is too close to the slide and using the coarse adjustment may scratch the objective lens.