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Cysteine can give two spots in chromatography due to its ability to exist in two different forms: the reduced form (cysteine) and the oxidized form (cystine), which is a dimer formed when two cysteine molecules link via a disulfide bond. These two forms can have different polarities and interactions with the stationary phase of the chromatography medium, leading to their separation and appearance as distinct spots on the chromatogram. Additionally, the pH of the mobile phase can influence the ionization state of cysteine, further contributing to the observed separation.
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What are the different types of chromatography?
there are different types like gas chrom. and thinlayer chrom Answer: There are two types of chromatography:liquid chromatography gas chromatography
How do you set up paper chromatography?
To set up paper chromatography, begin by cutting a strip of chromatography paper and marking a baseline about 1-2 cm from one end using a pencil. Next, apply small spots of the sample solution along the baseline, ensuring they are spaced apart. Then, place the paper in a developing chamber with a shallow layer of solvent, ensuring the baseline is above the solvent level. Finally, cover the chamber to prevent evaporation and allow the solvent to rise up the paper, separating the components of the sample.
An experiment was performed in lab that was supposed to produce aspirin. How could chromatography be used to tell if aspirin was actually the product of the reaction?
Chromatography could be used to separate the components of the reaction mixture and identify if aspirin is present by comparing the retention time of the product to that of a known aspirin standard. If the retention time matches, it indicates the presence of aspirin in the reaction mixture. Additionally, chromatography can help determine the purity of the aspirin product by analyzing the intensity of the peak corresponding to aspirin.
Where do hot spots originate?
Hot spots begin at where 2 plates move apart
What solution is C7H11NO7S?
4-nitro-2-toluenesulfonic acid dihydrate.
What are 3 types of chromatography?
1. thin -layer chromatography 2. gas chromatography 3. liquid chromatography
What are the different types of chromatography?
there are different types like gas chrom. and thinlayer chrom Answer: There are two types of chromatography:liquid chromatography gas chromatography
Why you are using 2 mercaptoethanol in mes cell culture?
Mercaptoethanol is a thiol compound that is involved in the reduction of substances and prevents the oxidation of cysteine to cystine, thus increasing cysteine activity. It acts as an antioxidant by itself and indirectly by increasing intracellular gluthathione and cysteine activity
Was chromatography used in World War 2?
yes
What are the differences between column and thin layer chromatography?
In chromatographic terms, TLC has great advantages over the other chromatography modes, such as Liquid Chromatography (LC), Column Chromatography (CC), Gas Chromatography (GC) and High Pressure Liquid Chromatography (HPLC).TLC's advantages are: (1) the ability to perform multiple analyses simultaneously; (2) speed and ease for scouting separation conditions, such as optimum solvent mixtures.
Where do you find all of the stones?
1.on the diving spots near mossdeep 2.give it to the guy's house above mossdeep
What are four ways to seperate mixtures?
1. Gravity, normal or with centrifugation. 2. Chemical precipitation. 3. Chromatography. 4. Electrophoresis. 1. Gravity, normal or with centrifugation. 2. Chemical precipitation. 3. Chromatography. 4. Electrophoresis.
An experiment was performed in lab that was supposed to produce aspirin. How could chromatography be used to tell if aspirin was actually the product of the reaction?
Chromatography could be used to separate the components of the reaction mixture and identify if aspirin is present by comparing the retention time of the product to that of a known aspirin standard. If the retention time matches, it indicates the presence of aspirin in the reaction mixture. Additionally, chromatography can help determine the purity of the aspirin product by analyzing the intensity of the peak corresponding to aspirin.
Where are the dig spots on Fable 2?
Your dog helps to find the dig spots in fable 2.
What is the third peak in a gas chromatography analysis of the dehydration of 2-methylcyclohexanol?
water
If a lady bug has 2 spots does it mean its 2 years old?
The number of spots identifies the type of ladybug. As ladybugs age, the color of the spots fade.
Where do hot spots originate?
Hot spots begin at where 2 plates move apart
