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Endonucleases are used in biotechnology because they are able to be applied to a specific part of a DNA sequence and will cut the DNA only in these locations. Exonucleases on the other hand have the following issues:
1) An exonuclease will digest and entirely consume DNA/RNA. As opposed to cut a specific location of DNA, they will eat the entire strand if they are not stopped.
2) They will only begin digestion at the ends of the DNA/RNA molecule. Circular plasmid DNA will not be affected by an exonuclease, because there are no beginnings or ends to the DNA sequence.
3) More over, as mentioned in (2) they will only begin digestion at the ends of the DNA sequences, thus they are much less accurate in where they begin cutting the DNA/RNA.
Because of this lack of control of exonucleases, they are difficult to use and generally not used in biotechnological techniques.
What else can I help you with?
What is the molecule that caused transformation in Griffith's mouse experiment?
The molecule that caused transformation in Griffith's mouse experiment was DNA. When heat-killed S strain bacteria (which had DNA) were mixed with live R strain bacteria (which did not), the DNA from the dead S strain bacteria was able to transfer genetic information to the live R strain bacteria, making them pathogenic.
What forms the rungs of the ladd r of DNA?
The base pairs form the rungs of the ladder.
What the letters does NOT represent one of the possible bases in the DNA structure?
The letter R does not represent one of the four possible bases in DNA. The four bases in DNA are adenine (A), thymine (T), cytosine (C), and guanine (G).
What is full form of DNA and rna?
Deoxiribose nuclic acid RNA ribos nuclic acid
What are the steps in RFLP?
Isolate DNA from the sample of interest. Digest the DNA with a restriction enzyme to cut it into fragments. Separate the fragments by size using gel electrophoresis. Transfer the fragments to a membrane and hybridize with a labeled probe to visualize the DNA fragments.
What is difference between recombinant DNA technology and Polymerase chain reaction?
r DNA technology is technology of creating new combination of DNA. While pcr is one of techniques used in r DNA technology for amplification of perticuler DNA fragment
What are some bakugan DNA codes that r not used?
gule53fw42,xdrs294gd7
Is hormone not a product of biotechnology?
Hormones can be produced through biotechnology methods such as genetic engineering and recombinant DNA technology. These methods allow for the large-scale production of hormones that can be used in various applications, including medical treatments and agriculture.
What chemicals are used to cut DNA into fragments?
Restriction enzymes, also known as restriction endonucleases, are used to cut DNA into fragments by recognizing specific DNA sequences and cleaving the phosphate backbone at these sites. These enzymes are crucial in molecular biology for techniques such as DNA cloning, gene editing, and DNA fingerprinting.
What technology begins with the letter R?
Robotic surgery is a modern technology. It begins with the letter R.
What technology word begins with the letter r?
Robotic surgery is a technology. It begins with the letter r.
Show the method to remove oil hydrocarbon from seeds on basis of r DNA technology and chemistry of oil?
It can be done by removing gene for oil synthesis from seeds by recombinant DNA technology or genetic engineering. - First the genes for formation of oil in the seed is identified and appropriate genesare removed with the help of enzyme restriction endonuleases. - This DNA is treated with DNA ligases to make seed with RNA at the broken ends. -The cells wih modified DNA is grown aseptically in a nutrient medium and will differntiate into a new plant whose seeds will not have oil in them.
Suggest amethode to remove oil from seeds based on recombinant DNA technology?
the constituents of oil is glycerol and fatty acids.using r-DNA tech.one can obtain oilless seeds by preventing the synthesis of glycerol or fatty acids.this is done by removing the specific gene responsible for the synthesis.
What has the author R Satyanarayana written?
R. Satyanarayana has written: 'Information technology and its facets' -- subject(s): Information technology
What are the six r's in food technology?
Reduce, rethink, refuse, recycle. reuse and repair are the 6 r's in technology according to the BBC.
Is alein DNA real?
Aleins r not realllll
When was Dr. B. R. Ambedkar National Institute of Technology created?
Dr. B. R. Ambedkar National Institute of Technology was created in 1987.
