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Why do you use buffers in separation of serum proteins by electrophoresis?
Buffers are used in electrophoresis to maintain a constant pH, which helps to separate serum proteins based on their charge. By keeping the pH stable, the proteins retain their charges and migrate towards the electrodes at different rates, allowing for separation based on size and charge.Buffers also help maintain an optimal environment for the proteins to remain stable and active during the electrophoresis process.
What is the purpose of immunoelectrophoresis?
Immunoelectrophoresis aids in the diagnosis and evaluation of the therapeutic response in many disease states affecting the immune system. It is usually requested when a different type of electrophoresis, called a serum protein.
Plasma minus clotting proteins?
Plasma minus clotting proteins is called serum. Serum is the liquid portion of blood that remains after blood has clotted and the clotting factors have been removed. Serum is used in various medical tests to measure different components in the blood.
What is the purpose of emulsifying the sample serum in staining procedure?
To promote the cells adhering to the glass slide since they can't be heat fixed. When preparing a capsule stain you have to aseptically add organisms and emulsify with a loop.
What tubes can be used to collect a serum specimen?
To collect a serum specimen, you can use a serum separator tube (SST), which typically contains a gel that facilitates the separation of serum from blood cells during centrifugation. Other options include plain red-top tubes, which do not contain any additives. Both types of tubes should be allowed to clot before centrifugation to ensure optimal serum yield.
Some viva voce questions for electrophoresis?
1. WHAT IS ELECTROPHORESIS AND WHAT ARE THE IMPORTANTAPPLICATIONS OF ELECTROPHORESIS?Ans. Movement of charged particle in the electric field either towards cathode or anode whensubjected to an electric current is called electrophoresis.The following factors influence the movement of particles during the electrophoresis.(a) Electric current.(b) Net charge of the particle.(c) Size and shape of the particle.(d) Type of supporting media.(e) Buffer solution.Important Applications of ElectrophoresisThe technique of electrophoresis is used to separate and identify the(i) Serum proteins(ii) Serum lipoproteins(iii) Blood hemoglobins2. WHAT ARE THE DIFFERENT TYPES OF ELECTROPHORESIS?Ans. (a) Moving boundary electrophoresis: This technique was first introduced by TISELIUS in 1937(b) Zone electrophoresis: In this type of electrophoresis different types of supporting mediaare used. These are;(a) Paper electrophoresis(i) Whatman filter paper(ii) Cellulose acetate(b) Gel electrophoresis(i) Agarose.(ii) Polyacrylamide gel (used for the separation of isoenzymes).(iii) SDS-PAGE.(iv) Iso-electric focussing (proteins seperated in a medium possessing a stable pH gradient).(v) Immuno electrophoresis (for the separation of immunoglobulins).
How long does it take to get results from serum protein electrophoresis test?
According to webmd.com, it takes 2-3 days.
Why do you use buffers in separation of serum proteins by electrophoresis?
Buffers are used in electrophoresis to maintain a constant pH, which helps to separate serum proteins based on their charge. By keeping the pH stable, the proteins retain their charges and migrate towards the electrodes at different rates, allowing for separation based on size and charge.Buffers also help maintain an optimal environment for the proteins to remain stable and active during the electrophoresis process.
How long do results take for a serum protein electrophoresis test?
Results for a serum protein electrophoresis test typically take about 1 to 3 days to be reported. The timeframe can vary depending on the laboratory's workload, the specific testing method used, and whether additional tests are needed for further analysis. Patients are usually informed by their healthcare provider once the results are available.
What has the author Neil S Harris written?
Neil S. Harris has written: 'Multiple myeloma and related serum protein disorders' -- subject(s): Electrophoresis, Myeloma Proteins, Analysis, Blood Proteins, Blood Protein Electrophoresis, Multiple Myeloma, Blood
What color tube is done for spep?
A lavender-top tube is commonly used for performing a Serum Protein Electrophoresis (SPEP) test. This tube contains the anticoagulant EDTA.
What is the purpose of immunoelectrophoresis?
Immunoelectrophoresis aids in the diagnosis and evaluation of the therapeutic response in many disease states affecting the immune system. It is usually requested when a different type of electrophoresis, called a serum protein.
How separated bands of human serum in a sds page can be recognised?
it could be recognized using a staining day that used to stain proteins in the sample it will separate several band according to molecular weight
Why did Arne Wilhelm Kaurin Tiselius win The Nobel Prize in Chemistry in 1948?
The Nobel Prize in Chemistry 1948 was awarded to Arne Tiselius for his research on electrophoresis and adsorption analysis, especially for his discoveries concerning the complex nature of the serum proteins.
What is lacking antibodies of a specific type in serum?
Serum is serum
What causes plasma cells to produce antibodies?
Yes. Blood plasma is blood serum with the fibrinogens. Serum includes all proteins not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances (e.g., drugs and microorganisms).
Plasma minus clotting proteins?
Plasma minus clotting proteins is called serum. Serum is the liquid portion of blood that remains after blood has clotted and the clotting factors have been removed. Serum is used in various medical tests to measure different components in the blood.
