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Why is it important that the Fehling's solution for use as a reagent by freshly mixed?
Fehling's solution contains copper sulfate and alkaline tartrate, which can deteriorate upon prolonged exposure to air. Freshly mixed Fehling's solution ensures the copper ions are in their active state for proper oxidation reactions in detecting reducing sugars, providing accurate results. Deterioration of the solution over time can lead to false negative test results.
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Why should benzidine reagent be freshly prepared?
Benzidine reagent should be freshly prepared to ensure accuracy and reliability of the results. It is prone to oxidation and degradation over time, which can lead to false positive or negative test outcomes. Freshly prepared benzidine reagent will provide the most accurate and consistent results in tests.
What reagent is used to test for unsaturated hydrocarbons?
This reagent is bromine in solution.
What happen when you react sugar like glucose and fructose with tollen' s reagent and the Fehlings solution?
Glucose oxidizes very quickly, and creates a silver mirror layer between the glucose solution and the Tollens' reagent. This is because of how the ketose reacts and reduces the silver molecules in Tollen's reagent.
What reagent is used to detect glucose in a solution?
The Benedict's reagent is commonly used to detect the presence of glucose in a solution. This reagent changes color from blue to green, yellow, orange, or red, depending on the amount of glucose present.
What is the color when fehling a and fehling b are being mixed?
When Fehling A & B are mixed in equal quantities... Fehlings Reagent is formed which is DEEP BLUE in colour Hope that answers your question! :)
What chemical formula for Fehlings reagent?
The Fehling A solution contain copper sulfate.The Fehling B solution contain sodium potassium tartrate and sodium hydroxide.
Why ketone do not react with Fehlings solution and Tollens reagent?
Ketones do not react with Fehling's solution or Tollens' reagent because they lack the free aldehyde group necessary for these reactions to occur. Both Fehling's solution and Tollens' reagent depend on the presence of the aldehyde group to participate in redox reactions that lead to the formation of a colored precipitate. Without this aldehyde group, ketones do not undergo these reactions.
Why should benzidine reagent be freshly prepared?
Benzidine reagent should be freshly prepared to ensure accuracy and reliability of the results. It is prone to oxidation and degradation over time, which can lead to false positive or negative test outcomes. Freshly prepared benzidine reagent will provide the most accurate and consistent results in tests.
Is honey a reducing sugar or non reducing?
yes because honey is a monosaccharide All monosaccharides reduce weak oxidizing agents such as Cu2+ in fehlings's reagent.
What is a positive result for the fehlings test?
A positive result for the Fehling's test indicates the presence of a reducing sugar such as glucose or fructose in the solution. This is shown by the formation of a brick-red precipitate when the Fehling's reagent is added and heated with the sugar solution.
What reagent is used to detect protein in a solution?
biuret reagent
What reagent is used to test for unsaturated hydrocarbons?
This reagent is bromine in solution.
What is fehlings solution?
Fehling solution "A" is copper sulphate solution and Fehling solution "B" is a solution of sodium potassium tartrate and NaOH.
Reaction of benzaldehyde with Schiff's reagent?
The Schiff reagent is a product of Fuchsine or Pararosaniline. The Schiff reagent is used to test for aldehydes. Benzaldehyde is added to the decolorized Schiff reagent and a purple/magenta color appears.
What happen when you react sugar like glucose and fructose with tollen' s reagent and the Fehlings solution?
Glucose oxidizes very quickly, and creates a silver mirror layer between the glucose solution and the Tollens' reagent. This is because of how the ketose reacts and reduces the silver molecules in Tollen's reagent.
Why is it important not to pipette a reagent solution directly from bottle of a reagent stock solution?
Pipetting directly from a reagent stock solution can lead to contamination of the stock solution. By introducing potential contaminants back into the stock solution, it can lead to inaccurate results in future experiments and compromise the integrity of the stock solution for other experiments. It's best practice to use a separate vessel to aliquot the required amount of reagent before transferring to the experiment.
What are the components of tollens reagent?
Tollens reagent is composed of silver nitrate solution, ammonia solution, and sodium hydroxide solution. Silver ions in the solution are reduced to silver metal, forming a silver mirror on the inside of a test tube when aldehydes are present.
