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What is the working volume in a 500ml shake flask?
The working volume in a 500ml shake flask is typically around 20-25% less than the total flask volume, so it would be around 375-400ml. This allows for proper aeration and mixing of the culture without overflowing during shaking.
How do clean a beaker?
Acid washing it should do. I assume you are in a lab...in which case a 1:1 Hydrochloric Acid solution can be poured into the flask. DON'T FORGET TO PUT GOGGLES AND GLOVES ON. Put the lid on. Shake the flask and turn it to ensure all surfaces of the glass inside are being washed with the acid. Then, give it a good triple rinse with de-ionized water. If you are just at home, pour in some white vinegar and let it sit for a while. That should break up anything that's inside.
What is the principle of shake-flask extraction?
Shake-flask extraction is a liquid-liquid extraction technique used to separate compounds based on their solubility in two immiscible liquids, typically water and an organic solvent. In this process, a mixture containing the target compound is placed in a flask along with the organic solvent, and the flask is shaken to promote mixing. The target compounds preferentially dissolve in one of the phases, allowing for their separation from the other components in the mixture. After shaking, the two phases separate, and the desired compound can be collected from the phase in which it is more soluble.
How do you make 9 Grams per liters solution of NaCl?
Because you asked how to make a 9 g/L solution of NaCl rather than a 9.0 g/L, or a 9.00 g/L solution, to make a 9 g/L solution of NaCl you first weigh out 9 g of dry NaCl. The weight, actually mass, of the NaCl needs to be 9 g plus or minus 1 g. Next, add the 9 g of NaCl to less than one liter of water, perhaps 25 or 50 mL less. Stir or shake the flask very well for several minutes after all of the NaCl has dissolved, then bring the total volume up to nearly 1 L. Stir or shake the flask very well again, then add water if necessary to ensure that the total volume is one liter.
What is shake flask fermentation?
Shake Flask fermentation is a cheapest ans simplest technique to grow bacteria or fungi, aerobically, in small volumes of nutrient broth. The broth is poured into erlenmeyer flasks equipped with cotton-wool stoppers, and autoclaved. After cooling, some microbes are "seeded" into the flask, and it is placed on a Shaker machine. The shaking agitates the content and so ensures aeration, so that the microbes could breathe.
What are design aspects of shake flask fermentation?
Design aspects of shake flask fermentation include selecting appropriate flask volume and shape, ensuring proper sealing to prevent contamination, choosing an appropriate stirring mechanism and speed, controlling temperature and aeration levels, and monitoring and adjusting pH and nutrient levels as needed. These aspects are crucial for optimizing microbial growth and product yield in shake flask experiments.
What is the working volume in a 500ml shake flask?
The working volume in a 500ml shake flask is typically around 20-25% less than the total flask volume, so it would be around 375-400ml. This allows for proper aeration and mixing of the culture without overflowing during shaking.
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informações sobre um frasco volumétrico de PFA (perfluoroalquilo) de alta pureza, com capacidade de 1000 ml e tampa de rosca, Classe A, para análise de isótopos utilizando ICP-MS. Esse tipo de frasco é ideal para garantir a integridade das amostras devido à sua resistência a contaminantes. Considerações: Material: O PFA é altamente resistente a produtos químicos e temperaturas, o que o torna adequado para análises sensíveis. Classe A: Garante a precisão do volume, importante para medições exatas. Antiaderente: Facilita a transferência e minimiza a perda de amostras.
How do clean a beaker?
Acid washing it should do. I assume you are in a lab...in which case a 1:1 Hydrochloric Acid solution can be poured into the flask. DON'T FORGET TO PUT GOGGLES AND GLOVES ON. Put the lid on. Shake the flask and turn it to ensure all surfaces of the glass inside are being washed with the acid. Then, give it a good triple rinse with de-ionized water. If you are just at home, pour in some white vinegar and let it sit for a while. That should break up anything that's inside.
What is the principle of shake-flask extraction?
Shake-flask extraction is a liquid-liquid extraction technique used to separate compounds based on their solubility in two immiscible liquids, typically water and an organic solvent. In this process, a mixture containing the target compound is placed in a flask along with the organic solvent, and the flask is shaken to promote mixing. The target compounds preferentially dissolve in one of the phases, allowing for their separation from the other components in the mixture. After shaking, the two phases separate, and the desired compound can be collected from the phase in which it is more soluble.
Why is it necessary to shake an oil and vinegar salad dressing before it to a salad?
It is necessary to shake an oil and vinegar salad dressing before adding it to a salad because they separate easily.
How do you make 9 Grams per liters solution of NaCl?
Because you asked how to make a 9 g/L solution of NaCl rather than a 9.0 g/L, or a 9.00 g/L solution, to make a 9 g/L solution of NaCl you first weigh out 9 g of dry NaCl. The weight, actually mass, of the NaCl needs to be 9 g plus or minus 1 g. Next, add the 9 g of NaCl to less than one liter of water, perhaps 25 or 50 mL less. Stir or shake the flask very well for several minutes after all of the NaCl has dissolved, then bring the total volume up to nearly 1 L. Stir or shake the flask very well again, then add water if necessary to ensure that the total volume is one liter.
Is it necessary to take a protein shake before your workout?
No, but it will help
What is shake flask fermentation?
Shake Flask fermentation is a cheapest ans simplest technique to grow bacteria or fungi, aerobically, in small volumes of nutrient broth. The broth is poured into erlenmeyer flasks equipped with cotton-wool stoppers, and autoclaved. After cooling, some microbes are "seeded" into the flask, and it is placed on a Shaker machine. The shaking agitates the content and so ensures aeration, so that the microbes could breathe.
Why is it necessary to shake a bottle of salad dessing?
Because when it settles the oil in it separates and sits in the bottom so you have to shake it to emulsify it again.
What is the difference between a dry shake and a wet shake when it comes to cocktail preparation?
A dry shake involves shaking cocktail ingredients without ice first, then shaking again with ice. This helps create a frothier texture. A wet shake involves shaking ingredients with ice from the start, resulting in a colder and more diluted drink.
How to clean a flask effectively?
To clean a flask effectively, first rinse it with warm water. Then, fill it with a mixture of warm water and dish soap, and shake it well. Use a bottle brush to scrub the inside, and rinse thoroughly. For tough stains, use a mixture of baking soda and water or white vinegar. Let it sit for a few hours before rinsing again. Finally, air dry the flask upside down.
