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What is the recommended protocol for preparing a protease inhibitor cocktail for cell lysis?

To prepare a protease inhibitor cocktail for cell lysis, it is recommended to mix multiple protease inhibitors in a buffer solution to prevent protein degradation. The cocktail should be added to the cell lysate immediately before use to ensure maximum effectiveness in preserving protein integrity.


What is the role of PMSF in protein extraction?

PMSF is a protease inhibitor. During the protein extraction, the proteases present in the cell lysate may digest the disered proteins, to prevent this PMSF is added!


What is the difference between lysis and lysate?

Lysis is the physical breakdown of a cell membrane, releasing its contents, while lysate is the resulting cell contents released after lysis. Lysis refers to the process of breaking open cells, whereas a lysate is the mixture of cellular components released from the broken cells.


What are protease enymes?

The protease enzyme helps in the breakdown of proteins present in a cell


What blocks HIV binding to host cell receptors?

Protease inhibitors.


Which components of HIV functions are release after of the virion from the infected cell?

protease


Which is a type of white blood cell protease macrophage placenta or lipase?

macrophage


Is protease a white blood cell?

No. A protease is an enzyme (i.e. a chemical), used in the digestion of proteins. White blood cells are called leucocytes and there are several different kinds such as lymphocytes.


How is protease related to AIDS?

The third class of antiretroviral drugs developed against HIV were the protease inhibitors. These work far back in the life cycle of HIV, after host cell integration but before budding. These drugs affect the enzyme protease, which is used to cut up the HIV protein to be packaged into virions. When the cell produces HIV proteins, the raw material is in a long connected string. The enzyme protease acts as a "scissor" to cut up the string into the protein for each virion. Protease inhibitors prevent protease from doing this. They resemble pieces of the protein string that protease usually cuts. This disrupts the cutting process, which prevents the chain from being cut into small pieces, which prevents HIV from making copies of itself.


What is protease inhibition?

Protease Inhibitors are a class of medications used to treat patients with the HIV virus. They are designed to prevent an already infected cell from reproducing (therefore inhibiting the virus from copying itself).


The opposite of combine?

Disect, lyse or Lysate.


What is protease inhibition therapy?

Protease Inhibitors are a class of medications used to treat patients with the HIV virus. They are designed to prevent an already infected cell from reproducing (therefore inhibiting the virus from copying itself).