0
In case you are talking about Polymerase chain reaction;
you melt the double strain from each other by raising the temperature. By lowering the temperature, DNA will melt together again. This would happen with the complement strain or with primers. But due to the length of the primer it will bind the matching sequence a lot faster than the complement strain. this is also balanced by strict temperature regulations during a PCR cycle.
* and ofcourse you dont replicate anything if the whole complement strain attaches!
What else can I help you with?
What role does the primase play in replication?
Primase is the enzyme responsible for synthesizing short RNA primers that provide a starting point for DNA synthesis by DNA polymerase during DNA replication. These primers serve as a foundation for the attachment of nucleotides that will form the new DNA strand. In essence, primer synthesis by primase initiates the replication process by allowing DNA polymerase to extend the primer with new DNA nucleotides.
What enzymes assembles the complementary nucleotides bases during replication?
During DNA replication, the enzyme DNA polymerase assembles complementary nucleotide bases. It adds nucleotides to the growing DNA strand by matching them with their complementary bases on the template strand. Additionally, RNA primase synthesizes a short RNA primer that provides a starting point for DNA polymerase to begin replication.
When does DNA replication take place?
DNA replication occurs during the synthesis phase of interphase of the cell cycle.
What does the synthesis of a new strand of DNA begin?
Following the initiation of DNA replication, the first step is the synthesis of a short RNA primer.
Okazaki fragments are joined together by?
During DNA replication Okazaki fragments are joined together by DNA polymerase. Remember that Okazaki fragments start with an RNA primer so RNAse H is need to remove the primer follwed by DNA plymerase to add nucleotides and finally DNA ligase to seal the single strand nick.
What role does the primase play in replication?
Primase is the enzyme responsible for synthesizing short RNA primers that provide a starting point for DNA synthesis by DNA polymerase during DNA replication. These primers serve as a foundation for the attachment of nucleotides that will form the new DNA strand. In essence, primer synthesis by primase initiates the replication process by allowing DNA polymerase to extend the primer with new DNA nucleotides.
What must occur before DNA replication can take place?
Before DNA replication can take place, the DNA molecule must unwind and separate into two strands, a process catalyzed by enzymes. This separation into single strands allows for the complementary base pairing to occur during replication. Additionally, a primer made of RNA is needed to initiate the process by providing a starting point for DNA polymerase to add nucleotides.
Does DNA replication take place in the cytoplasm of eukaryotic cells?
No, DNA replication takes place in the nucleus of eukaryotic cells. The nucleus contains the necessary enzymes and machinery for DNA replication to occur accurately.
What is the process involved in the replication of the DNA leading strand?
During DNA replication, the leading strand is synthesized continuously in the 5' to 3' direction by DNA polymerase. The process begins with the unwinding of the double helix by helicase, which creates a replication fork. Primase then synthesizes a short RNA primer, which is extended by DNA polymerase III. The RNA primer is later replaced with DNA by DNA polymerase I. The process continues until the entire leading strand is replicated.
Where does DNA replication takes place?
DNA replication takes place in NUCLEUS
Why RNA primers are needed during DNA replication and what is their fate?
DNA polymerase cannot begin the synthesis of new DNA.To synthesis a new strand of DNA ,RNA primer is required.The complementary RNA nucleotides,that are added opposite to the single strand of parent DNA are the RNA primer.
What enzymes assembles the complementary nucleotides bases during replication?
During DNA replication, the enzyme DNA polymerase assembles complementary nucleotide bases. It adds nucleotides to the growing DNA strand by matching them with their complementary bases on the template strand. Additionally, RNA primase synthesizes a short RNA primer that provides a starting point for DNA polymerase to begin replication.
Where does DNA replication take place?
DNA replication takes place in the nucleus of a cell.
What is the process called when DNA synthesis takes place during interphase?
Replication is when DNA makes more DNA
Two major enzymes used during DNA replication?
Two major enzymes used during DNA replication are DNA polymerase, which synthesizes new DNA strands by adding nucleotides in a complementary manner, and DNA helicase, which unwinds the DNA double helix to expose the template strands for replication.
When does DNA replication take place?
DNA replication occurs during the synthesis phase of interphase of the cell cycle.
Why doesn't RNA polymerase require a primer for transcription?
RNA polymerase does not require a primer for transcription because it can initiate the process on its own by recognizing specific DNA sequences called promoters. This allows RNA polymerase to bind to the DNA and start synthesizing RNA without the need for a primer like DNA polymerase does during DNA replication.
