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How do you calculate molecular weight in electrophoresis?

To calculate the molecular weight of a protein in electrophoresis, you would use a standard curve generated with protein standards of known molecular weights run on the same gel. By plotting the migration distance of the standard proteins against their known molecular weights, you can then determine the molecular weight of your protein of interest based on its migration distance on the gel in comparison to the standard curve.


Does low molecular weight heparin cross the placenta?

Standard heparin, an effective treatment for antepartum thromboembolic disease, is thought to be safe for the fetus since it does not cross the placenta. Recently, a number of low molecular weight heparins have been prepared which have been shown to produce less bleeding than standard heparin for an equivalent antithrombotic effect in experimental animals. These observations suggest that the low molecular weight heparins may also provide superior antithrombotic therapy in antepartum thromboembolic disease. However, it is not known whether the low molecular weight heparins cross the placenta. To determine this, we examined the pharmacokinetics of 125I-labelled standard heparin and a low molecular weight heparin, and their anticoagulant effects in mother and fetus, using a pregnant sheep model. Catheters were inserted into maternal and fetal femoral arteries at 108-119 d gestation (term: 147 d). 1-3 days later the mothers were given a bolus i.v. injection of 5000 anti-Xa units of 125I-labelled standard heparin or low molecular weight heparin, CY 222. Nine serial blood samples were collected over 4 h from both mother and fetus for measurements of radioactivity, anti-Xa activity (chromogenic) and activated partial thromboplastin times. When therapeutic levels of standard and CY 222 heparins were achieved in the mother, there was no detectable radioactivity or anticoagulant effect in the fetus. We conclude that standard heparin and the low molecular weight CY 222 do not cross the placenta in the pregnant sheep.


What is the molecular weight of malondialdehyde?

The molecular weight of malondialdehyde (C3H4O2) is 72.06 g/mol.


What is the molecular weight of serotonin?

The molecular weight of serotonin is approximately 176.203 g/mol.


What is the Molecular weight of magnesium bicarbonate?

MgCO3 has a molar mass (molecular weight) of 84.3139 g/mol.

Related Questions

Definition of primary STANDARD in pharmaceuticals?

the compounds which are having high molecular weight,easiliy available,chemially stable


How do you calculate molecular weight in electrophoresis?

To calculate the molecular weight of a protein in electrophoresis, you would use a standard curve generated with protein standards of known molecular weights run on the same gel. By plotting the migration distance of the standard proteins against their known molecular weights, you can then determine the molecular weight of your protein of interest based on its migration distance on the gel in comparison to the standard curve.


Why borax is a good primary standard?

Borax is a good primary standard because it is a hydrated salt with a known chemical formula and molecular weight that can be easily determined. It can be dried to a constant weight and is stable in air, making it reliable for accurate titrations. Additionally, borax is readily available, affordable, and dissolves easily in water.


WHY IS BORAX CONSIDERED A PRIMARY STANDARD?

Borax is considered a primary standard because it is a solid compound with a high degree of purity, a high molecular weight, and stable chemical properties. These characteristics make it suitable for accurately measuring the concentration of acids or bases in a titration procedure.


Why primary and secondary standard solutions are needed in analysis.?

Primary standards have some peculiar propertiesThey are nonsensitive to atmospheric oxygenThey have known formula and molecular weightThey are usually high molecular weight compoundsThey have a constant concentration /uniform composition for a long period of timeThey are powerful reactantsSecondary standards areInfluenced by atmosphere/environmentConcentration change over timeUsually powerful reactantsUsually cheap & easy to useZeryawkal Ergetie(Msc in pharmaceutical Analysis )


Can HNO3 be used as a primary standard?

No, HNO3 cannot be used as a primary standard because it is not a stable compound and is prone to atmospheric reactions and decomposition. Primary standards need to be stable, pure, have high molecular weight, and be easily accessible in a highly pure form.


What is primary standard solution?

A primary standard solution is a highly purified compound that is used as a reference material for accurately determining the concentration of another solution through titration. It is typically used in titration experiments due to its known high purity and stability.


Is EDTA considered as a primary standard?

No. Chemical compounds have to fulfill a number of requirements before they can be classified and used as a primary standard: A primary standard in chemistry is a reliable, readily quantified substance. Features of a primary standard include: 1. High purity 2. Stability (low reactivity) 3. Low hygroscopicity and efflorescence 4. High solubility (if used in titration) 5. High equivalent weight 6. Non-toxicity 7. Ready and cheap availability Edta and particularly the sodium salts of EDTA which are frequently used do not fulfill the first requirement. Therefore the solution of EDTA has to be standardised against a known and accepted primary standard.


What is the molecular weight of chymotrypsin?

It has a molecular weight of 21,600 Daltons


What is the molecular weight and molecular formula of potassium humate?

molecular structur C9H8K2O4molecular weight: 258.35


Why is non hygroscopic important for primary standard?

Then it is possible to measure accurately the weight of it, so you know exactly how many moles of the primary standard you have. When it would have been hygroscopic then your actual mass of the standard is lower than the measured, caused by the extra weight of water.


What is a primary standard?

A primary standard solution is generally a solution prepared and certified by an authorized and specialized institution (for example: NIST - National Institute for Standards and Technology).