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When doing reading on a spectrophotometer, the sample being studied is either a color change or a precipitated compound, depending on the wavelength that it is being read. If it is a precipitated compound and it has a very high concentration, then you run the risk of the light being used to measure the absorbance not going through. In which case you have total absorbance but it is inaccurate in helping you determine the concentration of your sample because you are unsure where the concentration limit is for that wavelength, and your sample could possibly be able to absorb more. In which case you still can't calculate the concentration of the sample.
What else can I help you with?
What are the components of ultra violet spectrophotometer?
are you taking forensics class in creede?spectrophotometry is analizing the color of a materials flame when burned to find how much energy it has and determine what exact substance it is.there should be a diagram in the book if you are asking the components that make up the spectrophotometer
How to fix concentration of RS by HPLC?
Concentration of sample= estimated LOQ concentration (µg/mL) x 1/desired LOQ (%) x 100LOQ should be Equal to or less than 0.05% of that of test concentration. Response of the impurity should be NLT 2000 at LOQ level for better precision.
Why test for carbon dioxide should be perfoed immediately after collection of water sample?
Testing for carbon dioxide in a water sample should be performed immediately after collection to ensure accurate results, as CO2 levels can change due to processes like diffusion and gas exchange with the atmosphere. Delays may lead to alterations in concentration due to the sample's exposure to air, temperature variations, or biological activity. Immediate testing helps maintain the integrity of the sample and provides a true representation of its original condition.
Should you rinse the pH meter between readings?
The electrode must be carefully rinsed.
What condition should use the standard addition method?
The standard addition method is typically used in analytical chemistry when analyzing samples with unknown concentrations, where a known amount of standard solution is added to the sample to create a series of solutions with different concentrations. This method is particularly useful when the matrix of the sample interferes with other quantitative methods.
What should be done if a spectrophotometer reports an absorbance that is too high?
If a spectrophotometer reports an absorbance that is too high, the solution may be too concentrated. Diluting the solution or using a lower concentration sample can help correct the issue. Additionally, checking for any potential errors in the calibration or measurement process is recommended.
What should be done if a spectrophotometer reports an absorbance that is too high and potentially affecting the accuracy of the results?
If a spectrophotometer reports a high absorbance that may impact result accuracy, recalibrate the instrument, check for any contamination in the sample or cuvette, and dilute the sample if necessary to obtain a more accurate reading.
What wavelength should be used in a spectrophotometer to obtain accurate and reliable measurements?
The wavelength used in a spectrophotometer should be specific to the substance being measured to obtain accurate and reliable measurements.
What are the components of ultra violet spectrophotometer?
are you taking forensics class in creede?spectrophotometry is analizing the color of a materials flame when burned to find how much energy it has and determine what exact substance it is.there should be a diagram in the book if you are asking the components that make up the spectrophotometer
What is absorbance vs concentration?
Absorbance is a measure of the amount of light absorbed by a sample at a specific wavelength, typically measured using a spectrophotometer. Concentration is the amount of a substance present in a unit volume of a solution, often expressed in moles per liter (M). The relationship between absorbance and concentration is governed by Beer's Law, which states that absorbance is directly proportional to concentration and path length.
A random sample should be taken from?
A random sample should be taken from an entire population.
How to fix concentration of RS by HPLC?
Concentration of sample= estimated LOQ concentration (µg/mL) x 1/desired LOQ (%) x 100LOQ should be Equal to or less than 0.05% of that of test concentration. Response of the impurity should be NLT 2000 at LOQ level for better precision.
How does increasing the sample size affect the sample error of the mean?
It should reduce the sample error.
Characteristics of a good sample?
1. the sample should be representative thus carefully selected. 2. the sample should be adequate thus significant enough.
How do you ensure less sample bias?
The sample should be selected randomly.
How long should a meat thermometer be left in the oven to ensure accurate temperature readings?
A meat thermometer should be left in the oven for about 10-15 seconds to ensure accurate temperature readings.
If a blood sample should be collected 2 hours postprandial when should the phlebotomist collect the sample?
2 hours after a meal.
