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If your mother is homozygous at D1S80 with a repeat of 200 base pairs and your father is homozygous at D1S80 with a repeat length of 400 pairs what will the length of your fragments be?
You inherit two alleles, one maternal and one paternal. Because both parents are homozygous, each only has one variation to pass down. This means that any offspring will be heterozygous and have one allele with a repeat of 200base pairs and one with 400bp.
Is the fan-shaped pile of broken rock fragments at the base of a steep bedrock slope or cliff?
The fan-shaped pile of broken rock fragments at the base of a steep bedrock slope or cliff is known as a talus slope or talus cone. This debris results from the weathering and erosion of the bedrock above, where gravity causes the rock fragments to accumulate at the base of the slope.
What is gel electrophoresis used for?
used to separate macromolecules, either nucleic acids or proteins, on the basis of size, electric charge, and other physical properties. Separating strands of DNA
Why gel red can stain DNA fragments?
GelRed is a fluorescent dye that is designed to bind to DNA by intercalating between the base pairs. This binding causes the DNA to fluoresce under UV light, making it visible in a gel electrophoresis setting. The staining ability of GelRed allows for the visualization of DNA fragments within the gel.
Fragments of DNA that have complementary sticky ends?
These fragments are called cohesive ends. They have short, single-stranded overhangs that can base pair with complementary overhangs on another DNA fragment. This allows for the fragments to be easily ligated together to form a larger DNA molecule.
If your mother is homozygous at D1S80 with a repeat of 200 base pairs and your father is homozygous at D1S80 with a repeat length of 400 pairs what will the length of your fragments be?
You inherit two alleles, one maternal and one paternal. Because both parents are homozygous, each only has one variation to pass down. This means that any offspring will be heterozygous and have one allele with a repeat of 200base pairs and one with 400bp.
How can one determine the number of base pairs in gel electrophoresis?
In gel electrophoresis, the number of base pairs in a DNA sample can be determined by comparing the distance the DNA fragments travel on the gel to a standard ladder of known base pair sizes. The size of the DNA fragments can be estimated by their migration distance relative to the ladder, allowing for the determination of the number of base pairs in the sample.
What is a DNA size standard and why is it used in gel electrophoresis?
You may be referring to the DNA ladder used in gel electrophoresis. The ladder is a collection of DNA fragments of known size (e.g. 100, 500, 1000, 2000, 5000, 10000 base pairs) so that if it is loaded beside the samples, it can offer a 'ruler' that can be used to determine the size of the fragments in the samples.
Why is gel electrophoresis important?
To separate and analyze DNA fragments and protein fragments by weight. If you have digested some bacterial DNA, for instance, then you can tell by running the fragmented DNA in the gel whether you have digested the correct base length.
Agarose gels are used to study what size of DNA fragments?
When the electric charge is applied, the bigger, unligated peices stay near the well because they are too large to move as fast as the smaller pieces. The smaller fragments are farther from the well since they move more easily through the gel.
What could be done to resolve two fragments that are nearly the same base-pair length and did not appear on the electrophoresis gel?
You could use other enzymes or use a higher percetage of agarosa to make your gel (so they will have a better chance of separating).
How long are the base pairs of Okazaki fragments?
Okazaki fragments are typically around 100-200 base pairs long in prokaryotes and around 1000-2000 base pairs long in eukaryotes.
Why can gel electrophoresis separate dna fragments?
The markers are of a specific size (in numbers of base pairs). By comparing the rates of migration of the various markers with the rates of migration of the gene/genes you are separating, you can get an estimate of the size of the fragments you are interested in. They also let you know if you need more or less separation. If the smaller fragments run off of the gel, you'll know that you need to run if for a shorter time or with a smaller voltage.
What is the purpose of ethidium bromide?
Ethidium bromide is an intercalating agent that attaches itself between the helix of a DNA. Because the ethidium molecule lights up when illuminated by an ultraviolet light, it is used often in biochemistry laboratories so that fragment of DNA that has been separated by gel can be visualized.
Is the fan-shaped pile of broken rock fragments at the base of a steep bedrock slope or cliff?
The fan-shaped pile of broken rock fragments at the base of a steep bedrock slope or cliff is known as a talus slope or talus cone. This debris results from the weathering and erosion of the bedrock above, where gravity causes the rock fragments to accumulate at the base of the slope.
What is gel electrophoresis used for?
used to separate macromolecules, either nucleic acids or proteins, on the basis of size, electric charge, and other physical properties. Separating strands of DNA
Is there a base word for debris?
Yes, the base word for debris is "debr-" which comes from the French word "débris" meaning remains or fragments.
