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Blunt or sticky ends are not a problem for the ligase, the thing is, the enzyme used should be the same to cut the insert and the vecotr(which may be a single or double enzyme but same!)

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Destruction of a kidney stone by ultrasonic waves traveling through water?

Ultrasonic waves are used to break kidney stones into smaller fragments, making them easier to pass through the urinary tract. The waves are produced by a device called a lithotripter, and are directed at the stone from outside the body while the patient is immersed in a water bath. The high-energy waves cause the stone to fragment without the need for surgery.


Do the smallest fragments in a gel electrophoresis end up on top or bottom?

Depending on where the died fragments start, the smallest parts end up way on the other side. The gel acts as a filter and the electrical current acts as... the current to push the fragments through the gel. Being that they're small... those fragments have an easier time getting through the gel. The bigger fragments are closer to where the fragments started cause they're big and have a harder time going through the gel. Eventually you should have like areas in the gel that look cool and CSI like, as if you were testing for DNA samples. Sadly that may not always happen, being as... well this is reality and not show biz. Good luck though on your next/first attempt.


What causes the bands on your DNA gels?

During gel electrophoresis, the DNA moves along the agarose gel to the positive side of the box, and after a certain amount of time, the smaller DNA fragments travel the farthest (because they have an easier time navigating the pores of the gel) and so on, leaving behind a series of bands comprised of similar-sized DNA fragments.


What is the function of agarose gel electrophoresis?

Agarose gel electrophoresis is used to separate DNA fragments based on size. When an electric current is applied to the gel, DNA molecules move through the pores of the gel at different rates depending on their size, allowing for visualization and analysis of DNA fragments in a sample.


What are the objectives of agarose?

The agarose gel acts as a matrix that slows down the dna segments as they move to the opposite charged end of the gel. A larger segment will have a tougher time moving through the gel, while a smaller segment will move faster because it is easier to move it through the gel.

Related Questions

Why is it so important to be able to amplify DNA fragments when studying genes?

Amplifying DNA fragments is important when studying genes because it allows researchers to create multiple copies of a specific DNA sequence, making it easier to analyze and study the genetic information contained within that fragment. This process, known as polymerase chain reaction (PCR), helps scientists to identify and understand the functions of genes, as well as to detect genetic variations and mutations that may be associated with diseases or other traits.


What is corticated ossific fragments?

Corticated ossific fragments are pieces of bone that are surrounded by a layer of cortical bone, making them more well-defined and easier to distinguish on imaging studies like X-rays or CT scans. These fragments are usually associated with fractures or injuries to bones.


How can one reconnect with lost family members?

The best way to reconnect with lost family members is to have them meet you in a public place like a restaurant. If they have not seen you in many years the public atmosphere put them at ease and make making that initial connection easier.


Destruction of a kidney stone by ultrasonic waves traveling through water?

Ultrasonic waves are used to break kidney stones into smaller fragments, making them easier to pass through the urinary tract. The waves are produced by a device called a lithotripter, and are directed at the stone from outside the body while the patient is immersed in a water bath. The high-energy waves cause the stone to fragment without the need for surgery.


What is an advantage of working with short DNA fragments?

There are several advantages to working with short DNA fragments. These include being amplified better viaÊPCR technology, having better stability so that they do not break apart, and rarely degrading.


Do the smallest fragments in a gel electrophoresis end up on top or bottom?

Depending on where the died fragments start, the smallest parts end up way on the other side. The gel acts as a filter and the electrical current acts as... the current to push the fragments through the gel. Being that they're small... those fragments have an easier time getting through the gel. The bigger fragments are closer to where the fragments started cause they're big and have a harder time going through the gel. Eventually you should have like areas in the gel that look cool and CSI like, as if you were testing for DNA samples. Sadly that may not always happen, being as... well this is reality and not show biz. Good luck though on your next/first attempt.


What does Taping the DNA onto large paper simulate?

Taping DNA onto large paper simulates DNA electrophoresis, a process used to separate and visualize DNA fragments based on size. By laying out the DNA fragments in a straight line, it allows for easier analysis and comparison of different DNA samples.


What causes the bands on your DNA gels?

During gel electrophoresis, the DNA moves along the agarose gel to the positive side of the box, and after a certain amount of time, the smaller DNA fragments travel the farthest (because they have an easier time navigating the pores of the gel) and so on, leaving behind a series of bands comprised of similar-sized DNA fragments.


Why is the failure rate of a tubal ligation higher when done during a Cesarean section?

During a c-section your body still has pregnancy hormones and it makes it easier for the tubes to begin to heal themselves and reconnect


How do you replace solenoid in 65 Chevy C10 pickup?

It's usually easier to remove the starter from the truck and do the work on a bench. Remove the bolts that hold the solenoid, put new solenoid in it's place. Reconnect.


Can bone fragments in gums cause swelling?

Yes- the oral surgeon or denist can remove them. They may come back, as soon as they are removed, so you may make a few trips. Its easier if they preatrude thru the gums.


How does HCD fragmentation contribute to the identification of complex molecules in mass spectrometry analysis?

HCD fragmentation in mass spectrometry helps break down complex molecules into smaller fragments, making it easier to identify and analyze them.