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Franz Ziehl(Bacteriologist) andFrederick Neelson(pathologist) are the two scientists who discovered Ziehl-Neelson stain or acid-fast stain.Mycobacterium tuberculosis is an organism with high lipid conent in its cell wall because of which acid fast stain is used. 1.smearpreparation 2.flood with cabolfuchsin under continuous heating 3.wash with acid alcohol(20%hci:ethanol) 4.flood methelene blue.wash with water observe under microscope
What else can I help you with?
Can dyes or other methylene blue be used for direct staining?
Crystal violet, basic fuchsin, and safranin are all dyes which can be used in direct staining because they are cationic which means that they are positively charged. These dyes which are positively charged will react to the bacterial cell wall because the cell wall is negatively charged resulting in a basic stain.
What is the minimum amount of protein that can appear in SDS PAGE gel?
Although this would in part depend the size of the protein being separated and stained (for some staining methods), the largest factor that determines sensitivity of SDS-PAGE is the type of staining method used: - If staining is done with coomassie brilliant blue, the limit of detection claimed by most suppliers is 50 ng. In my experience, 100-1000 ng is more accurate for proteins of 20-30 kDa. - If staining is done with silver stain, the limit of detection is much lower (or higher sensitivity). Manufacturers usually claim that 5-50 ng of protein can be visualized, but in my experience 50 ng is the lower limit for average sized proteins (20-30 kDa). - If visualization is accomplished with an enzyme immunoassay, the limit of detection is lower still, as low as 0.1-1 ng (100-1000 pg). - If visualization is accomplished with radio immunoassay the limit of detection becomes much lower again, easily to the picogram level (0.001 ng).
What is staining rack?
A staining rack is a laboratory tool used to hold and organize slides during the staining process. It typically has grooves or slots where slides can be securely placed to prevent them from moving or touching each other while applying different staining solutions. Staining racks help in efficient and uniform staining of multiple slides at once.
Why is safrinin used in gram staining?
as a couterstain
Is carbol fuchsin the same as safranin?
No. safranin is the classic stain used in gram staining. Concentrated Carbol Fushin is mainly used for the ZN staining procedure to stain organisms such as Vibrio cholerae and Cryptosporidium. Diluted Carbol Fushin can however be used as a replacement counterstain for Safranin in the gram stain.
What is the use of coplin jar?
A Coplin jar is used in laboratory settings to hold and process multiple microscope slides at the same time. It is commonly used for staining procedures, such as the Gram stain, where multiple slides need to be immersed in various staining reagents simultaneously.
Used for error detection?
Used for error detection
Can dyes or other methylene blue be used for direct staining?
Crystal violet, basic fuchsin, and safranin are all dyes which can be used in direct staining because they are cationic which means that they are positively charged. These dyes which are positively charged will react to the bacterial cell wall because the cell wall is negatively charged resulting in a basic stain.
Which staining technique is used to identify the simple stain(s)?
The staining technique used to identify simple stains is called the simple staining technique.
What is the primary stain used in Gram staining?
The primary stain used in Gram staining is crystal violet.
Uses of staining rack?
used to hold the glass glides while staining them. (:
What can be used for a simple stain procedure?
Perhaps Gram Staining? Steps are as follows: 1. Crystal Violet, 2. Iodine, 3. Decolorizer, 4. Safrinin
What is the purpose of differential staining?
differential staining is a staining technique used to stain colorless bacteria against a dark background.
What mordant is used in the process of gram staining?
The mordant used in the process of gram staining is called crystal violet.
What is the minimum amount of protein that can appear in SDS PAGE gel?
Although this would in part depend the size of the protein being separated and stained (for some staining methods), the largest factor that determines sensitivity of SDS-PAGE is the type of staining method used: - If staining is done with coomassie brilliant blue, the limit of detection claimed by most suppliers is 50 ng. In my experience, 100-1000 ng is more accurate for proteins of 20-30 kDa. - If staining is done with silver stain, the limit of detection is much lower (or higher sensitivity). Manufacturers usually claim that 5-50 ng of protein can be visualized, but in my experience 50 ng is the lower limit for average sized proteins (20-30 kDa). - If visualization is accomplished with an enzyme immunoassay, the limit of detection is lower still, as low as 0.1-1 ng (100-1000 pg). - If visualization is accomplished with radio immunoassay the limit of detection becomes much lower again, easily to the picogram level (0.001 ng).
What is the function of staining jar?
There are several uses for a staining jar. In microscopy, it is used for staining tissues and cells for slides. After being stained with dyes or stains, the specimens can also be placed in the jar to look for certain aspects.
What is staining rack?
A staining rack is a laboratory tool used to hold and organize slides during the staining process. It typically has grooves or slots where slides can be securely placed to prevent them from moving or touching each other while applying different staining solutions. Staining racks help in efficient and uniform staining of multiple slides at once.
