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Endopeptidases act on the peptide bonds within protein chains, breaking them into smaller polypeptides or peptides, which increases the surface area for subsequent digestion. This initial cleavage facilitates the efficient action of exopeptidases, which further break down these smaller peptides into free amino acids or dipeptides. By acting first, endopeptidases ensure a more effective and rapid overall digestion process, optimizing nutrient absorption in the digestive system. Additionally, this sequential action helps maintain a balanced rate of digestion, preventing potential overload on the exopeptidases.

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