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Allolactose is a molecule that binds to the lac repressor protein in E. coli, causing it to detach from the lac operator region in the lac operon. This allows RNA polymerase to bind to the promoter and transcribe the genes necessary for lactose metabolism. Ultimately, this process enables the bacteria to use lactose as an energy source.

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What are the roles of allolactose in the lac operon and tryptophan in trp operon?

allolactose acts as an inducer


How does induction by IPTG differ from induction by lactose via allolactose?

Lactose is eventually metabolized in the cell to allolactose, which binds to the lacI repressor, leading to the induction of the lac promoter (or derivatives of the lac promoters). The allolactose is also eventually metabolised. IPTG does essentially the same as allolactose, however, it does not get metabolized. Moreover, since lactose gets transported in the cell by the lacY gene product and metabolized to allolactose by the lacZ gene product, lactose cannot be used in lacZY- strains for induction. IPTG, however, can be used since it does not require the two genes.


What is the function of allolactose in the operations of the lac operon?

allolactose acts as an inducer


What is allolactose and how does it work?

Allolactose isca sugar, isomeric with lactose, that is the true inducer of the lac operon. An agent capable of activating specific genes. A molecule that inhibits the action of the repressor of an operon, preventing it from freely binding with the operator gene and disabling its function.


What is lac operon in E. coli?

The lac operon is a place on the DNA that binds to the DNA to stop the production of the RNA sequence to code for lactase (breaks down lactose) or binds to lactase to let the production of the RNA sequence to proceed.


What is the difference between the induction mechanisms of IPTG and lactose in gene expression regulation?

IPTG is a synthetic inducer that directly binds to the lac repressor protein, preventing it from blocking the lac operon. Lactose, on the other hand, is a natural inducer that is converted into allolactose, which then binds to the lac repressor to release it from the lac operon.


When is the lactose operon likely to be transcribed?

The lactose operon is likely to be transcribed in the absence of glucose and presence of lactose. When glucose is low and lactose is available, the inducer molecule allolactose binds to the repressor protein, causing it to be released from the operator region and enabling RNA polymerase to transcribe the operon.


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