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What else can I help you with?
Does lactococcus lactis grow in bile esculin?
Lactococcus lactis is a lactic acid bacteria commonly found in dairy products and does not typically grow in high levels in bile esculin. This medium is mainly used to differentiate enterococci, Streptococcus bovis, and Group D Streptococcus based on their ability to hydrolyze esculin in the presence of bile.
What is the purpose of sodium azide in the Bile Esculin test?
Sodium azide is included in the Bile Esculin test to inhibit the growth of Gram-negative organisms, which could interfere with the test results. Sodium azide helps to maintain a selective environment that favors the growth of group D streptococci, the target organisms for the test.
What are the differences between staphylococcus aureus and enterococcus faecalis biochemical tests?
E.coli is a gram negative rod mostly isolated from urine while staph is a gram positive cocci. You can differentiate them by color. E. Coli is Pink which is negative while Staph is Purple with is positive.
Can horse chestnuts be eaten?
Horse chestnuts are not safe for human consumption, as they contain a toxic compound called esculin. Consuming horse chestnuts can cause nausea, vomiting, and other serious health issues. It is important to avoid eating them.
What are some common acid-base artificial indicators?
Alizarin is an orange dye present in the root of the madder plant; it was used to dye wool in ancient Egypt, Persia, and India. In an 0.5% alcohol solution, alizarin is yellow at pH 5.5 and red at pH 6.8. Several synthetic modifications of alizarin are also used as acid/base indicators.Cochineal is an acid-base indicator made from the bodies of dried female cochineal insects, found in Mexico and Central America. You'll have to grind about 70,000 insects to make one pound of dry indicator. The powder is about 10% carminic acid, which is yellow in acidic solution, and deep violet in alkaline solution. Cochineal solutions are not used much as acid/base indicators these days.Curcumin, or tumeric yellow, is a natural dye found in curry powder. It turns from yellow at pH 7.4 to red at pH 8.6.Esculin is a fluorescent dye that can be extracted from the leaves and bark of the horse chestnut tree. You'll need to shine a black (ultraviolet) light on the indicator to get the full effect. Esculin changes from colorless at pH 1.5 to fluorescent blue at pH 2.
Is red buckeye edible?
They aren't something you should be eating. At the same time, I don't believe they're poisonous or anything like that. However according to a post on the attached link Buckeyes (also known as horse chestnuts) are in fact poisonous. It indicates that the leaves, sprouts, and seed (nut) contain a poisonous glycoside called esculin. The entry goes on to list symptoms such as twitching muscles, weakness, loss of coordination, dilation of pupils, vomiting, diarrhea, depression, paralysis and stupor. So it doesn't sound like it will kill you outright.. but I'm no doctor to say for certain. The answer cited two professionals from North Carolina State University (Angela M. Fraser, Ph.D., Associate Professor/Food Safety Specialist & Carolyn J. Lackey, Ph.D., R.D., L.D.N., Professor/Food and Nutrition Specialist) as having provided the information above.
What is the fucntion of oxgall?
Oxbile (Oxgall) is dehydrated bile for use in preparing microbiological culture media. Oxbile is manufactured from large quantities of fresh bile by rapid evaporation of the water content. Bile is composed of fatty acids, bile acids, inorganic salts, sulfates, bile pigments, cholesterol, mucin, lecithin, glycuronicacids, porphyrins, and urea. The use of Oxbile insures a regular supply of bile, and uniformity impossible to obtain with fresh materials. Oxbile is dehydrated fresh bile and prepared specifically for differentiation of bile tolerant microorganisms. A 10% solution of dehydrated bile is equivalent to a fresh bile solution. It is usually incorporated into media e.g., Bile Esculin Agar and Brilliant Green Bile Agar, used for the determination of enteric pathogens. Oxbile is also found in Littman Agar, a selective fungal medium. It is used as a selective agent for the isolation of Gram-negative microorganisms, inhibiting Gram-positive bacteria. The major composition of Oxbile is taurocholic and glycocholic acids.
Can you elimintate gram positive bacteria from a gram positive gram negative mixture with mild detergent treatment?
AnswerYou can use a "selective" medium that will inhibit the growth of Gram positive bacteria and only allow Gram negative bacteria to multiply. A medium which is commonly used for this is the McConkey agar which contains a crystal violet strain and allows only Gram negative cultures to grow.You can also eliminate Gram positive bacteria with antibiotics (e.g. ampicillin) provided that they are sensitive and not resistant.
Biochemical test for identification of gram positive bacteria?
the answer i would like to give. there are many biochemical tests for detection of various types of bacteria. 1. carbohydrate utilization tests (sugar test). - lactose , sucrose , mannitol , maltose , xylose and dextrose. 2. imvic test. it includes indole test , methyl red test , voges proskuer test , citrate utilization test. 3. nitrate reduction test. 4. urease test 5. triple sugar iron test. 6. lead acetate test. 7. mannitol motility test 8. O-F (oxidation - fermentation) test 9. aminoacid degradation test we can detect enterobacteriaecae group & other group of bacilli according to their charactristics by comparing with standard table.
