There are limitations to colorimetry. These limitations include similar colors that produce errors in results, a tighter wavelength band width, and interferences that can produce bad results in uncontrolled situations.
Some disadvantages to spectroscopy are that it is only effective at low analyte concentrations, it can be greatly influenced by stray light radiation, and that absorbance must be taken near the peak absorbance otherwise the value may vary greatly.
Indirect spectrophotometry methods such as colorimetric assays completely break down and destroy the proteins within the sample. They are rendered useless for further analysis or experimentation.
Flame photometry has many disadvantages. Perhaps the most difficult of these is the control of the many variables. The intensity from a flame is dependent on the flame temperature, the rate of flow of liquid into the flame, the pressure and rate of flow of fuel gases, and any of many other variables which affect the character of the flame or atomizing of the solution have a great effect. The experimental results are therefore only empirical.
wat r de disadvantages of using titration cus i ay got a clue nd i need help plzzzz!!!!
Requires lot of sample for analysis
esr spectroscopy hyper line
Spectroscopy originated through the study of visible light dispersed by a prism according to its wavelength. An instrument called a spectrometer is used in spectroscopy for producing spectral lines and measuring their wavelengths and intensities.
Molecules emit electromagnetic radiation in NMR spectroscopy.
Advantage: non-destructive to the sample Disadvantage: High detection limit
no,it is reflection spectroscopy
Mass spectrometry, UV/Vis spectroscopy, NMR spectroscopy CNMR spectroscopy, Infra red spectroscopy
Emission photo-spectroscopy and Absorption photo-spectroscopy.
Several variations of Raman spectroscopy have been developed.· Surface Enhanced Raman Spectroscopy (SERS)· Resonance Raman spectroscopy· Surface-Enhanced Resonance Raman Spectroscopy (SERRS)· Angle Resolved Raman Spectroscopy· Hyper Raman· Spontaneous Raman Spectroscopy (SRS)· Optical Tweezers Raman Spectroscopy (OTRS)· Stimulated Raman Spectroscopy· Spatially Offset Raman Spectroscopy (SORS)· Coherent anti-Stokes Raman spectroscopy (CARS)· Raman optical activity (ROA)· Transmission Raman· Inverse Raman spectroscopy.· Tip-Enhanced Raman Spectroscopy (TERS)· Surface plasmon polaritons enhanced Raman scattering (SPPERS)
Fluorescence spectroscopy is a type of spectroscopy that analyzes fluorescence from a provided sample. This uses a beam of light, often an ultraviolet light which then causes absorption spectroscopy to occur.
1 infra-red (UV-VIS) spectroscopy. 2 proton magnetic resonance spectroscopy. 3 carbon 13 magnetic resonoce spectroscopy.
Stephen G. Schulman has written: 'Fluorescence and phosphorescence spectroscopy' -- subject(s): Fluorescence spectroscopy, Phosphorescence spectroscopy 'Molecular Luminescence Spectroscopy'
Russell H Barnes has written: 'Laser spectroscopy for continuous combustion applications' -- subject(s): Raman spectroscopy, Fluorescence spectroscopy, Laser spectroscopy
disadvantages - radiation can ionize and damage cells and is very expensive to use. advantages - can go in lots of detail, and results are usually very clear
S. Svanberg has written: 'Atomic and molecular spectroscopy' -- subject(s): Atomic spectroscopy, Molecular spectroscopy
S. Wartewig has written: 'IR and Raman spectroscopy' -- subject(s): Infrared spectroscopy, Raman spectroscopy