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Depending on the microscope the steps differ but assuming you are using a light microscope (the only microscope you will use before university). The microscope must be set to the lowest magnification with the stage set as far way as possible (the platform the sample is held on). Then using the coarse focus (the larger knob) bring the stage towards the lens while looking though the eye piece when the sample comes into focus use the fine focus knob (the smaller knob) to bring the image to optimum focus. When this is achieved increases the magnification (by changing to a stronger lens) and using fine focus continue to focus the image and keep repeating the steps till a max focus is achieved. If more information is need let me know as there are more complicated techniques but this should be enough for before a university level depending on what country you are in.

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14y ago

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