No. Amtrak serves neither city.
It is 214.83 miles according to MapQuest.
Caryville, TN is a little over halfway but you don't have to get off the interstate.
about 67 miles
MapQuest estimates the driving time as 3 hours and 22 minutes.
175 miles
133 miles
175.92 miles
Nashville is larger in area. Louisville is larger in population.
3 fortnights and a bottle of rum
The highest elevation between Nashville, TN and Louisville, KY is located near Jellico, TN, at around 1,379 feet above sea level.
It is 210.48 miles according to MapQuest.
It is 133.58 miles according to MapQuest.