It's 4 stops on the Uptown E train, so it's about 5 to 10 minutes.
Walk west to Lexington Avenue and north to 53rd Street, and take the Downtown E train (of the A-C-E, the blue line) from Lexington Avenue-53rd Street to 34th Street-Penn Station.
Take the Uptown E train (of the A-C-E, the blue line) from 34th Street-Penn Station to 53rd Street-5th Avenue. Fifth Avenue is one block west of Madison Avenue.
To get from Penn Station to Worth Street, you can take the 1 subway line. Board the 1 train at 34th Street-Penn Station and travel downtown. Get off at the Chambers Street station, which is close to Worth Street. Alternatively, you could take the A or C train from 34th Street-Penn Station to Chambers Street for a similar route.
The fare is the same regardless of where you're going. See the Related Question below for directions.
Take the Uptown E train (of the A-C-E, the blue line) from 34th Street-Penn Station to 5th Avenue-53rd Street. Then walk 1 block west on 53rd Street to 6th Avenue.You'll know which way is west because 5th Avenue is the dividing line between the West and East Sides. So on the west side of 5th Avenue, the street sign will say "West 53rd Street," while on the east side of 5th Avenue, it will say "East 53rd Street."
The American Girl store is at the corner of 5th Avenue and 49th Street. Take the Uptown E train (of the A-C-E, the blue line) from 34th Street-Penn Station to 5th Avenue-53rd Street. Then walk 4 blocks down 5th Avenue to 49th Street.
No, the AirTrain takes you to and from the subway, and the subway takes you to and from Penn Station. Take the AirTrain to the Howard Beach Station, and get the Manhattan-bound A train (of the A-C-E, the blue line) to 34th Street-Penn Station.
To get from Penn Station to the Museum of Modern Art (MoMA), take the E subway line from Penn Station heading towards World Trade Center. Ride for two stops and transfer at 42nd Street-Port Authority Bus Terminal to the 7 train. Take the 7 train one stop to 5th Avenue. From there, it's a short walk to MoMA, located at 11 West 53rd Street.
To travel from LaGuardia Airport to Penn Station using the subway, you can take the M60 bus from the airport to the Astoria Blvd subway station. From there, you can transfer to the N or W train and take it to 34th Street-Herald Square station, which is near Penn Station.
Take the Uptown E train (of the A-C-E, the blue line) from 34th Street-Penn Station to 5th Avenue-53rd Street. Then walk 2 blocks south on 5th Avenue to St. Patrick's (on 5th Avenue between 51st and 50th Streets).
Take the Downtown 2 or 3 trains (of the 1-2-3, the red line) from 34th Street-Penn Station to Wall Street (at William Street).
To get from Penn Station to 54th Street in New York City, you can take the 1, 2, or 3 subway lines from 34th Street-Penn Station. Transfer at Times Square-42nd Street to the N, Q, R, or W trains heading downtown. Alternatively, you can also walk a few blocks to catch the E train at 34th Street-Penn Station, which will take you directly to 54th Street.