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Two significant legislative acts of the Assembly that impacted the Roman Catholic Church were the Civil Constitution of the Clergy (1790) and the Decree on the Separation of Church and State (1795). The Civil Constitution restructured the Catholic Church in France, making clergy employees of the state and requiring them to take an oath of loyalty, which led to widespread conflict and schism. The Decree on the Separation of Church and State established a clear boundary between religious and governmental authority, effectively diminishing the Church's influence in public life and governance.

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