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Chromatography
Chromatography is a set of techniques used to separate mixtures in a chemistry lab setting. The word chromatography means "color writing" and some of the first experiments in chromatography involved separating chlorophyll in plants.
649 Questions
What is gas-liquid chromatography?
It is used to check purity of gas. percentage of a gas in a mixed gas. I have seen it in Producer gas plant . It is used here to check % CO, %O2 in the gas . This total automated Gas Chromatograph gives Graphical Output to the PLC or Script chart Recorder in a plant.
What is locating agent used for?
Locating agent is a substance/liquid that help to separate and analyze colorless substances in chromatography by reacting with the colorless substance to form colored spots on the chromatogram.
What problems may arise in the use from a poorly packed liquid chromatography column?
The effect of packing a chromatographic column unevenly will cause the flow to be uneven down the column and lead to poor separation.
What is thermally unstable mean?
If a substance is thermally unstable it means it breaks down at high temperature. It cannot withstand high temperature.
What causes the reaction in chromatography?
In chromatography, the reaction is caused by the interaction between the molecules of the sample being separated and the stationary phase (such as paper or silica gel). This interaction leads to different components of the sample moving at different rates, resulting in separation based on their affinity for the stationary phase.
Rf values are used mainly for simplicity. These values are generally easier to use to obtain percentages between a couple objects instead of using values that are already established.
Should water content or LOD be subtracted from potency?
Whichever is higher in value should be subtracted
An assay is a scientific test or analysis used to measure the presence, amount, or activity of a substance or compound in a sample. Assays are commonly used in biology, chemistry, and medicine to study a wide range of biological and chemical processes.
Should an ionic crystal conduct electricity?
You would not expect them to as the ions are locked in place in a latticee. This is generally true there are however a few exceptions.
They are called fast ion conductors or solid electrolytes.
Examples:- Rubidium silver iodide is conductive at room temp. Lead fluoride was the first to be discovered.
What is the difference between vacuum chromatography and flash chromatography?
Flash chromatography uses pressure (under 10 psi) to pump solvent down a column at a rate faster than gravity would provide. Vacuum chromatography uses a vacuum at the bottom of the column to pull solvent through. Both can be performed with standard glass columns, but usually vacuum chromatography is done with a silica filled vacuum funnel instead as a rough purification technique.
The rf value is shorthand for the retention value of a substance. It is used in chromatography to determine the components of an unknown sample.
What are the differences between column and thin layer chromatography?
In chromatographic terms, TLC has great advantages over the other chromatography modes, such as Liquid Chromatography (LC), Column Chromatography (CC), Gas Chromatography (GC) and High Pressure Liquid Chromatography (HPLC).TLC's advantages are: (1) the ability to perform multiple analyses simultaneously; (2) speed and ease for scouting separation conditions, such as optimum solvent mixtures.
Does the temperature of the water change the boiling point?
The boiling point of water depends only on pressure, not ambient temperature. The only correlation between climate temperature and boiling point would come about from the fact that cold climates in temperate parts of the Earth are usually at higher elevations than warmer climates and therefore have lower atmospheric pressure.
What variable affect dissolving?
Several variables affect the rate of dissolving, including temperature, agitation (stirring), and particle size of the solute. Higher temperatures generally increase the solubility of solids in liquids by providing more energy for the solute particles to break apart. Agitation helps distribute solute particles more evenly throughout the solvent, enhancing interactions. Additionally, smaller particle sizes increase the surface area available for dissolution, leading to faster dissolving rates.
Why do colors travel different distances in chromatography?
Because photon gravitation differs to the light travel...Ps. future technologies will be used to capture photon gravitation with the precision of laser and crystal mirrors. This helps to accelarate present time or slowdown time captured in mirrors view.
What is the meaning of duplicates and triplicates?
Doing duplicates means doing the experiment / process two times.
Doing triplicates means doing the experiment / process three times.
How do you reveal the position of a colorless spot on the chromatogram?
By adding a specific reagent.
What is solvent for benzil to develop TLC and Rf?
A common solvent mixture for developing TLC plates with benzil as the compound is a mixture of ethyl acetate and hexane in a 1:1 ratio. This solvent system can help achieve good separation and clear Rf values for benzil on the TLC plate.
What is the use of calculating asymmetry factor in hplc?
The asymmetry factor in HPLC is used to assess the peak shape of a chromatographic peak. It is calculated by dividing the front part of the peak by the back part, providing information on the peak tailing or fronting. A symmetrical peak typically has an asymmetry factor close to 1, indicating good peak shape.
It is common in my field of working and there hv been always solutions to such situations. Friends, books, study, professionals, outsourcing, etc. as it suits the situation have been the solution.
Lignohumate is a type of organic compound derived from lignin, which is a complex polymer found in plant cell walls. It has been researched for its potential use as a soil conditioner and promoter of plant growth due to its ability to chelate nutrients and improve soil structure.
