A possible answer could be '4 chambers in the human heart'.
...to the e, to the e, to the c, to the r, to the e, d, i, t, free to the credit to the dot to the com, c'mon everybody grab your bike and sing along.
Why is a joker in a pack of cards?
The extra "Joker" card is believed to have been invented by American Euchre players who, when modifying the rules sometime during the 1860s, decided that an extra trump card was required. Originally he was called "The Best Bower" or "Imperial Bower," and then later "The Jolly Joker".
These Jokers, or extra cards, were first introduced into American packs around 1863, but took a little longer to reach English packs, in around 1880. One British manufacturer (Chas Goodall) was manufacturing packs with Jokers for the American market in the 1870s.
26 characters in the alphabet. Only true for the modern Roman alphabet.