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The purity of a culture of bacteria is important so it can test on that one type of bacteria. Gram staining can be good so you make sure everything in the streak plate is one color showing that it is gram positive and gram negative.
The rods will be gram negative, the cocci are gram positive: most likely, the slide was made from a mixed culture of bacteria, or the culture was contaminated.
the answer for that is he had no culture
Sounds pretty typical.
If a culture has been growing for 72 hours, it is likely that most of the bacterial cells would have undergone multiple rounds of cell division and may have varying degrees of cell wall synthesis. As a result, it would be challenging to predict a specific gram reaction without additional information or performing a Gram stain test on the culture.
A gram variable bacteria is a type of bacterium that does not consistently stain as either gram-positive or gram-negative. This variability can make it challenging to identify using traditional Gram staining techniques.
Gram-variable bacilli are rod-shaped bacteria that do not consistently take up Gram stain, appearing either Gram-positive or Gram-negative under a microscope. This variability can result from factors such as the age of the culture, the composition of the cell wall, or the presence of certain conditions during staining. Gram-variable bacilli can include various species, some of which are pathogenic and associated with human diseases. Examples include certain strains of Corynebacterium and Mycobacterium.
deffirent bacteria show different response to gram stainig procedure so bacteria are classified into two groups:a(gram positive bacteria)b(gram negitive bacteria)
Genetics, Age of culture, type of growth medium, and technique used could result in a gram-variable reaction
Typically, a negative result can be doubted, but a positive staining cannot be. This is because the vast majority of bacteria that do revert (not to say that the majority of bacteria DO revert) will go from positive to negative. This is due to a decreased average level of peptidoglycan synthesis in Agar culture, as opposed to natural function in vivo. The decreased level of peptidoglycan allows the ethanol/acetone decolorizer to more quickly flush the Crystal Violet from Gram+ cells, making them appear either weakly positive, or fully negative.
mids is $10 a gram beasters is $10 a gram fruit is $15 a gram kush is $20 a gram and up...hope i helped :)
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